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| - | ==Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome==
| + | #REDIRECT [[4v85]] This PDB entry is obsolete and replaced by 4v85 |
| - | <StructureSection load='3sfs' size='340' side='right' caption='[[3sfs]], [[Resolution|resolution]] 3.20Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[3sfs]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SFS FirstGlance]. <br>
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| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
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| - | <tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5OH:(2S)-AMINO[(4R,6S)-2-AMINO-6-HYDROXY-3,4,5,6-TETRAHYDROPYRIMIDIN-4-YL]ETHANOIC+ACID'>5OH</scene>, <scene name='pdbligand=DPP:DIAMINOPROPANOIC+ACID'>DPP</scene>, <scene name='pdbligand=KBE:BETA-LYSINE'>KBE</scene>, <scene name='pdbligand=UAL:(2Z)-2-AMINO-3-(CARBAMOYLAMINO)PROP-2-ENOIC+ACID'>UAL</scene></td></tr>
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| - | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sgf|3sgf]]</td></tr>
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| - | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b4375, JW5873, miaD, prfC, Termination Release Factor 3, tos ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
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| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sfs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sfs RCSB], [http://www.ebi.ac.uk/pdbsum/3sfs PDBsum]</span></td></tr>
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| - | <table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | The class II release factor RF3 is a GTPase related to elongation factor EF-G, which catalyzes release of class I release factors RF1 and RF2 from the ribosome after termination of protein synthesis. The 3.3 A crystal structure of the RF3.GDPNP.ribosome complex provides a high-resolution description of interactions and structural rearrangements that occur when binding of this translational GTPase induces large-scale rotational movements in the ribosome. RF3 induces a 7 degrees rotation of the body and 14 degrees rotation of the head of the 30S ribosomal subunit, and itself undergoes inter- and intradomain conformational rearrangements. We suggest that ordering of critical elements of switch loop I and the P loop, which help to form the GTPase catalytic site, are caused by interactions between the G domain of RF3 and the sarcin-ricin loop of 23S rRNA. The rotational movements in the ribosome induced by RF3, and its distinctly different binding orientation to the sarcin-ricin loop of 23S rRNA, raise interesting implications for the mechanism of action of EF-G in translocation.
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| - | Crystal structure of release factor RF3 trapped in the GTP state on a rotated conformation of the ribosome.,Zhou J, Lancaster L, Trakhanov S, Noller HF RNA. 2011 Dec 20. PMID:22187675<ref>PMID:22187675</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | ==See Also==
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| - | *[[Ribosome 3D structures|Ribosome 3D structures]]
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Escherichia coli]]
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| - | [[Category: Lancaster, L.]]
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| - | [[Category: Noller, H F.]]
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| - | [[Category: Trakhanov, S.]]
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| - | [[Category: Zhou, J.]]
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| - | [[Category: Ribosome-antibiotic complex]]
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| - | [[Category: Typeii release factor binding with ribosome]]
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