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| | ==Structure of the LSm657 Complex: An Assembly Intermediate of the LSm1 7 and LSm2 8 Rings== | | ==Structure of the LSm657 Complex: An Assembly Intermediate of the LSm1 7 and LSm2 8 Rings== |
| - | <StructureSection load='3swn' size='340' side='right' caption='[[3swn]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='3swn' size='340' side='right'caption='[[3swn]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3swn]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SWN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3swn]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SWN FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lsm7, SPCC285.12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]), lsm5, SPBC20F10.09 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]), lsm6, SPAC2F3.17c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [http://www.ebi.ac.uk/pdbsum/3swn PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [https://pdbe.org/3swn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [https://www.ebi.ac.uk/pdbsum/3swn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3swn ProSAT]</span></td></tr> |
| - | <table> | + | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/LSM7_SCHPO LSM7_SCHPO] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner. Probable component of the spliceosome. |
| - | The nuclear LSm2-8 (like Sm) complex and the cytoplasmic LSm1-7 complex play a central role in mRNA splicing and degradation, respectively. The LSm proteins are related to the spliceosomal Sm proteins that form a heteroheptameric ring around small nuclear RNA. The assembly process of the heptameric Sm complex is well established and involves several smaller Sm assembly intermediates. The assembly of the LSm complex, however, is less well studied. Here, we solved the 2.5 A-resolution structure of the LSm assembly intermediate that contains LSm5, LSm6, and LSm7. The three monomers display the canonical Sm fold and arrange into a hexameric LSm657-657 ring. We show that the order of the LSm proteins within the ring is consistent with the order of the related SmE, SmF, and SmG proteins in the heptameric Sm ring. Nonetheless, differences in RNA binding pockets prevent the prediction of the nucleotide binding preferences of the LSm complexes. Using high-resolution NMR spectroscopy, we confirm that LSm5, LSm6, and LSm7 also assemble into a 60-kDa hexameric ring in solution. With a combination of pull-down and NMR experiments, we show that the LSm657 complex can incorporate LSm23 in order to assemble further towards native LSm rings. Interestingly, we find that the NMR spectra of the LSm57, LSm657-657, and LSm23-657 complexes differ significantly, suggesting that the angles between the LSm building blocks change depending on the ring size of the complex. In summary, our results identify LSm657 as a plastic and functional building block on the assembly route towards the LSm1-7 and LSm2-8 complexes.
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| - | Structure of the LSm657 complex: an assembly intermediate of the LSm1-7 and LSm2-8 rings.,Mund M, Neu A, Ullmann J, Neu U, Sprangers R J Mol Biol. 2011 Nov 25;414(2):165-76. Epub 2011 Oct 6. PMID:22001694<ref>PMID:22001694</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| | ==See Also== | | ==See Also== |
| - | *[[Sm-like protein|Sm-like protein]] | + | *[[Sm-like protein 3D structures|Sm-like protein 3D structures]] |
| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Schizosaccharomyces pombe]] | + | [[Category: Large Structures]] |
| - | [[Category: Mund, M.]] | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: Neu, A.]] | + | [[Category: Mund M]] |
| - | [[Category: Neu, u.]] | + | [[Category: Neu A]] |
| - | [[Category: Sprangers, R.]] | + | [[Category: Neu U]] |
| - | [[Category: Ullmann, J L.]] | + | [[Category: Sprangers R]] |
| - | [[Category: Nuclear]]
| + | [[Category: Ullmann JL]] |
| - | [[Category: Rna]]
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| - | [[Category: Rna binding protein]]
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| - | [[Category: Rna metabolism]]
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| - | [[Category: Sm]]
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