3svl

Structural highlights

Function

CHRR_ECOLI Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr(6+) producing Cr(3+) and one electron to molecular oxygen without producing the toxic Cr(5+) species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V(5+), Mo(6+), methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.^{[1] [2] [3] [4]}

References

1. ↑ Ackerley DF, Gonzalez CF, Park CH, Blake R 2nd, Keyhan M, Matin A. Chromate-reducing properties of soluble flavoproteins from Pseudomonas putida and Escherichia coli. Appl Environ Microbiol. 2004 Feb;70(2):873-82. PMID:14766567
2. ↑ Ackerley DF, Barak Y, Lynch SV, Curtin J, Matin A. Effect of chromate stress on Escherichia coli K-12. J Bacteriol. 2006 May;188(9):3371-81. PMID:16621832 doi:http://dx.doi.org/10.1128/JB.188.9.3371-3381.2006
3. ↑ Barak Y, Ackerley DF, Dodge CJ, Banwari L, Alex C, Francis AJ, Matin A. Analysis of novel soluble chromate and uranyl reductases and generation of an improved enzyme by directed evolution. Appl Environ Microbiol. 2006 Nov;72(11):7074-82. PMID:17088379 doi:http://dx.doi.org/72/11/7074
4. ↑ Eswaramoorthy S, Poulain S, Hienerwadel R, Bremond N, Sylvester MD, Zhang YB, Berthomieu C, Van Der Lelie D, Matin A. Crystal Structure of ChrR-A Quinone Reductase with the Capacity to Reduce Chromate. PLoS One. 2012;7(4):e36017. Epub 2012 Apr 27. PMID:22558308 doi:10.1371/journal.pone.0036017