3uwx

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==Crystal structure of UvrA-UvrB complex==
==Crystal structure of UvrA-UvrB complex==
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<StructureSection load='3uwx' size='340' side='right' caption='[[3uwx]], [[Resolution|resolution]] 4.40&Aring;' scene=''>
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<StructureSection load='3uwx' size='340' side='right'caption='[[3uwx]], [[Resolution|resolution]] 4.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3uwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus Geobacillus] and [http://en.wikipedia.org/wiki/Geobacillus_sp._y412mc52 Geobacillus sp. y412mc52]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UWX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3uwx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_sp._Y412MC52 Geobacillus sp. Y412MC52]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UWX FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.398&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GYMC52_3203, uvrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=129337 Geobacillus]), GYMC52_3204, uvrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550542 Geobacillus sp. Y412MC52])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uwx RCSB], [http://www.ebi.ac.uk/pdbsum/3uwx PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwx OCA], [https://pdbe.org/3uwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uwx RCSB], [https://www.ebi.ac.uk/pdbsum/3uwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uwx ProSAT]</span></td></tr>
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<table>
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</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A0E0TG05_GEOS2 A0A0E0TG05_GEOS2] The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.[HAMAP-Rule:MF_00205]
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Nucleotide excision repair (NER) is used by all organisms to eliminate DNA lesions. We determined the structure of the Geobacillus stearothermophilus UvrA-UvrB complex, the damage-sensor in bacterial NER and a new structure of UvrA. We observe that the DNA binding surface of UvrA, previously found in an open shape that binds damaged DNA, also exists in a closed groove shape compatible with native DNA only. The sensor contains two UvrB molecules that flank the UvrA dimer along the predicted path for DNA, ~80 A from the lesion. We show that the conserved signature domain II of UvrA mediates a nexus of contacts among UvrA, UvrB and DNA. Further, in our new structure of UvrA, this domain adopts an altered conformation while an adjacent nucleotide binding site is vacant. Our findings raise unanticipated questions about NER and also suggest a revised picture of its early stages.
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Structure and mechanism of the UvrA-UvrB DNA damage sensor.,Pakotiprapha D, Samuels M, Shen K, Hu JH, Jeruzalmi D Nat Struct Mol Biol. 2012 Feb 5;19(3):291-8. doi: 10.1038/nsmb.2240. PMID:22307053<ref>PMID:22307053</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
*[[UvrABC|UvrABC]]
*[[UvrABC|UvrABC]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Geobacillus]]
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[[Category: Geobacillus sp. Y412MC52]]
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[[Category: Geobacillus sp. y412mc52]]
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[[Category: Large Structures]]
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[[Category: Jeruzalmi, D.]]
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[[Category: Jeruzalmi D]]
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[[Category: Pakotiprapha, D.]]
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[[Category: Pakotiprapha D]]
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[[Category: Abc atpase]]
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[[Category: Atp binding]]
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[[Category: Dna binding]]
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[[Category: Dna binding protein]]
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[[Category: Dna repair]]
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[[Category: Nucleotide excision repair]]
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[[Category: Uvra]]
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[[Category: Uvrb]]
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Current revision

Crystal structure of UvrA-UvrB complex

PDB ID 3uwx

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