3t4b

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==Crystal Structure of the HCV IRES pseudoknot domain==
==Crystal Structure of the HCV IRES pseudoknot domain==
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<StructureSection load='3t4b' size='340' side='right' caption='[[3t4b]], [[Resolution|resolution]] 3.55&Aring;' scene=''>
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<StructureSection load='3t4b' size='340' side='right'caption='[[3t4b]], [[Resolution|resolution]] 3.55&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3t4b]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T4B FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3t4b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepatitis_C_virus_ED43 Hepatitis C virus ED43] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T4B FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.55&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t4b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t4b RCSB], [http://www.ebi.ac.uk/pdbsum/3t4b PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t4b OCA], [https://pdbe.org/3t4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t4b RCSB], [https://www.ebi.ac.uk/pdbsum/3t4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t4b ProSAT]</span></td></tr>
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<table>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Translation of hepatitis C viral proteins requires an internal ribosome entry site (IRES) located in the 5' untranslated region of the viral mRNA. The core domain of the hepatitis C virus (HCV) IRES contains a four-way helical junction that is integrated within a predicted pseudoknot. This domain is required for positioning the mRNA start codon correctly on the 40S ribosomal subunit during translation initiation. Here, we present the crystal structure of this RNA, revealing a complex double-pseudoknot fold that establishes the alignment of two helical elements on either side of the four-helix junction. The conformation of this core domain constrains the open reading frame's orientation for positioning on the 40S ribosomal subunit. This structure, representing the last major domain of HCV-like IRESs to be determined at near-atomic resolution, provides the basis for a comprehensive cryoelectron microscopy-guided model of the intact HCV IRES and its interaction with 40S ribosomal subunits.
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Crystal structure of the HCV IRES central domain reveals strategy for start-codon positioning.,Berry KE, Waghray S, Mortimer SA, Bai Y, Doudna JA Structure. 2011 Oct 12;19(10):1456-66. PMID:22000514<ref>PMID:22000514</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bai, Y.]]
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[[Category: Hepatitis C virus ED43]]
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[[Category: Berry, K E.]]
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[[Category: Large Structures]]
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[[Category: Doudna, J A.]]
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[[Category: Synthetic construct]]
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[[Category: Mortimer, S A.]]
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[[Category: Bai Y]]
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[[Category: Waghray, S.]]
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[[Category: Berry KE]]
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[[Category: Four-way junction]]
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[[Category: Doudna JA]]
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[[Category: Hcv ires central domain]]
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[[Category: Mortimer SA]]
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[[Category: Pseudoknot]]
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[[Category: Waghray S]]
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[[Category: Rna]]
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Current revision

Crystal Structure of the HCV IRES pseudoknot domain

PDB ID 3t4b

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