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3tsi

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==Structure of the parainfluenza virus 5 (PIV5) hemagglutinin-neuraminidase (HN) stalk domain==
==Structure of the parainfluenza virus 5 (PIV5) hemagglutinin-neuraminidase (HN) stalk domain==
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<StructureSection load='3tsi' size='340' side='right' caption='[[3tsi]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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<StructureSection load='3tsi' size='340' side='right'caption='[[3tsi]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3tsi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Parainfluenza_virus_5 Parainfluenza virus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TSI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TSI FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3tsi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_virus_5_(strain_W3) Simian virus 5 (strain W3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TSI FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11207 Parainfluenza virus 5])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.651&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tsi OCA], [https://pdbe.org/3tsi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tsi RCSB], [https://www.ebi.ac.uk/pdbsum/3tsi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tsi ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tsi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tsi RCSB], [http://www.ebi.ac.uk/pdbsum/3tsi PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/HN_PIV5 HN_PIV5] Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity). Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.
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== Publication Abstract from PubMed ==
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Paramyxovirus entry into cells requires the fusion protein (F) and a receptor binding protein (HN, H or G). The multifunctional hemagglutinin-neuraminidase (HN) protein of some paramyxoviruses, besides functioning as the receptor (sialic acid) binding protein (hemagglutinin activity) and the receptor-destroying protein (neuraminidase activity), enhances fusion protein (F) activity, presumably by lowering the activation energy required for F to mediate fusion of viral and cellular membranes. Before or upon receptor binding by the HN globular head, F is believed to interact with the HN stalk. Unfortunately until recently none of the receptor binding protein crystal structures have shown electron density for the stalk domain. Parainfluenza virus 5 (PIV5) HN exists as a non-covalent dimer-of-dimers on the surface of cells, linked by a single disulfide bond in the stalk. Here we present the crystal structure of the PIV5-HN stalk domain at a resolution of 2.65 A revealing a four-helix bundle (4HB) with an upper (N-terminal) straight region and a lower (C-terminal) supercoiled part. The hydrophobic core residues are a mix of an 11-mer repeat and a 3-4 heptad repeat. To functionally characterize the role of the HN stalk in F-interactions and fusion, we designed mutants along the PIV5-HN stalk that are N-glycosylated to physically disrupt F-HN interactions. By extensive study of receptor binding, neuraminidase activity, oligomerization and fusion promoting functions of the mutant proteins we found a correlation between the position of the N-glycosylation mutants on the stalk structure and their neuraminidase activities as well as their abilities to promote fusion.
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Structure and mutagenesis of the parainfluenza virus 5 hemagglutinin-neuraminidase stalk domain reveals a four-helix bundle and the role of the stalk in fusion promotion.,Bose S, Welch BD, Kors CA, Yuan P, Jardetzky TS, Lamb RA J Virol. 2011 Oct 12. PMID:21994464<ref>PMID:21994464</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Hemagglutinin|Hemagglutinin]]
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*[[Hemagglutinin 3D structures|Hemagglutinin 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Exo-alpha-sialidase]]
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[[Category: Large Structures]]
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[[Category: Parainfluenza virus 5]]
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[[Category: Bose S]]
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[[Category: Bose, S.]]
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[[Category: Jardetzky TS]]
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[[Category: Jardetzky, T S.]]
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[[Category: Kors CA]]
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[[Category: Kors, C A.]]
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[[Category: Lamb RA]]
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[[Category: Lamb, R A.]]
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[[Category: Welch BD]]
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[[Category: Welch, B D.]]
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[[Category: Yuan P]]
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[[Category: Yuan, P.]]
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[[Category: 11-mer hydrophobic repeat]]
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[[Category: Ectodomain]]
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[[Category: Four stranded coiled coil]]
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[[Category: Four-helix bundle]]
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[[Category: Receptor binding protein]]
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[[Category: Viral protein]]
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Current revision

Structure of the parainfluenza virus 5 (PIV5) hemagglutinin-neuraminidase (HN) stalk domain

PDB ID 3tsi

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