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3t1q

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==MglA bound to GppNHp in complex with MglB==
==MglA bound to GppNHp in complex with MglB==
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<StructureSection load='3t1q' size='340' side='right' caption='[[3t1q]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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<StructureSection load='3t1q' size='340' side='right'caption='[[3t1q]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3t1q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T1Q FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3t1q]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T1Q FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3t12|3t12]], [[3t1o|3t1o]], [[3t1r|3t1r]], [[3t1s|3t1s]], [[3t1t|3t1t]], [[3t1v|3t1v]], [[3t1x|3t1x]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTHA1132 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus]), TTHA1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1q OCA], [https://pdbe.org/3t1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t1q RCSB], [https://www.ebi.ac.uk/pdbsum/3t1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t1q ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>
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</table>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t1q RCSB], [http://www.ebi.ac.uk/pdbsum/3t1q PDBsum]</span></td></tr>
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== Function ==
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<table>
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[https://www.uniprot.org/uniprot/Q5SJ83_THET8 Q5SJ83_THET8]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The bacterium Myxococcus xanthus uses a G protein cycle to dynamically regulate the leading/lagging pole polarity axis. The G protein MglA is regulated by its GTPase-activating protein (GAP) MglB, thus resembling Ras family proteins. Here, we show structurally and biochemically that MglA undergoes a dramatic, GDP-GTP-dependent conformational change involving a screw-type forward movement of the central beta2-strand, never observed in any other G protein. This movement and complex formation with MglB repositions the conserved residues Arg53 and Gln82 into the active site. Residues required for catalysis are thus not provided by the GAP MglB, but by MglA itself. MglB is a Roadblock/LC7 protein and functions as a dimer to stimulate GTP hydrolysis in a 2:1 complex with MglA. In vivo analyses demonstrate that hydrolysis mutants abrogate Myxococcus' ability to regulate its polarity axis changing the reversal behaviour from stochastic to oscillatory and that both MglA GTPase activity and MglB GAP catalysis are essential for maintaining a proper polarity axis.
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Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.,Miertzschke M, Koerner C, Vetter IR, Keilberg D, Hot E, Leonardy S, Sogaard-Andersen L, Wittinghofer A EMBO J. 2011 Aug 16. doi: 10.1038/emboj.2011.291. PMID:21847100<ref>PMID:21847100</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Gliding protein|Gliding protein]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Small monomeric GTPase]]
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Koerner, C.]]
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[[Category: Koerner C]]
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[[Category: Miertzschke, M.]]
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[[Category: Miertzschke M]]
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[[Category: Vetter, I R.]]
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[[Category: Vetter IR]]
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[[Category: Wittinghofer, A.]]
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[[Category: Wittinghofer A]]
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[[Category: Alpha/beta protein]]
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[[Category: Bacterial gtpase]]
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[[Category: Bacterial polarity]]
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[[Category: G domain containing protein]]
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[[Category: Gtpase activating protein]]
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[[Category: Homodimer]]
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[[Category: Hydrolase-signaling protein complex]]
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[[Category: Motility]]
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[[Category: Pole localisation]]
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Current revision

MglA bound to GppNHp in complex with MglB

PDB ID 3t1q

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