3t43

<StructureSection load='3t43' size='340' side='right' caption='[[3t43]], [[Resolution|resolution]] 1.95&Aring;' scene=''>

<table><tr><td colspan='2'>[[3t43]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T43 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T43 FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t43 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t43 RCSB], [http://www.ebi.ac.uk/pdbsum/3t43 PDBsum]</span></td></tr>

Methods for rapid and reliable design and structure prediction of linker loops would facilitate a variety of protein engineering applications. Circular permutation, in which the existing termini of a protein are linked by the polypeptide chain and new termini are created, is one such application that has been employed for decreasing proteolytic susceptibility and other functional purposes. The length and sequence of the linker can impact the expression level, solubility, structure and function of the permuted variants. Hence it is desirable to achieve atomic-level accuracy in linker design. Here, we describe the use of RosettaRemodel for design and structure prediction of circular permutation linkers on a model protein. A crystal structure of one of the permuted variants confirmed the accuracy of the computational prediction, where the all-atom rmsd of the linker region was 0.89 A between the model and the crystal structure. This result suggests that RosettaRemodel may be generally useful for the design and structure prediction of protein loop regions for circular permutations or other structure-function manipulations.

 

High-resolution structure prediction of a circular permutation loop.,Correia BE, Holmes MA, Huang PS, Strong RK, Schief WR Protein Sci. 2011 Nov;20(11):1929-34. doi: 10.1002/pro.725. Epub 2011 Sep, 30. PMID:21898647<ref>PMID:21898647</ref>

 

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<references/>

[[Category: Holmes, M A.]]

[[Category: Strong, R K.]]

[[Category: Transferase]]