3tw8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:15, 1 March 2024) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
 +
==GEF domain of DENND 1B in complex with Rab GTPase Rab35==
==GEF domain of DENND 1B in complex with Rab GTPase Rab35==
-
<StructureSection load='3tw8' size='340' side='right' caption='[[3tw8]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+
<StructureSection load='3tw8' size='340' side='right'caption='[[3tw8]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3tw8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TW8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TW8 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3tw8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TW8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TW8 FirstGlance]. <br>
-
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2fol|2fol]], [[1yzn|1yzn]]</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DENND1B, C1orf218, FAM31B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RAB35, RAB1C, RAY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tw8 OCA], [https://pdbe.org/3tw8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tw8 RCSB], [https://www.ebi.ac.uk/pdbsum/3tw8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tw8 ProSAT]</span></td></tr>
-
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tw8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tw8 RCSB], [http://www.ebi.ac.uk/pdbsum/3tw8 PDBsum]</span></td></tr>
+
</table>
-
<table>
+
== Function ==
-
<div style="background-color:#fffaf0;">
+
[https://www.uniprot.org/uniprot/DEN1B_HUMAN DEN1B_HUMAN] Guanine nucleotide exchange factor (GEF) for RAB35. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. May play a role in clathrin-mediated endocytosis.<ref>PMID:20154091</ref> <ref>PMID:20937701</ref>
-
== Publication Abstract from PubMed ==
+
-
Rab GTPases are key regulators of membrane traffic pathways within eukaryotic cells. They are specifically activated by guanine nucleotide exchange factors (GEFs), which convert them from their "inactive" GDP-bound form to the "active" GTP-bound form. In higher eukaryotes, proteins containing DENN-domains comprise a major GEF family. Here we describe at 2.1-A resolution the first structure of a DENN-domain protein, DENND1B-S, complexed with its substrate Rab35, providing novel insights as to how DENN-domain GEFs interact with and activate Rabs. DENND1B-S is bi-lobed, and interactions with Rab35 are through conserved surfaces in both lobes. Rab35 binds via switch regions I and II, around the nucleotide-binding pocket. Positional shifts in Rab residues required for nucleotide binding may lower its affinity for bound GDP, and a conformational change in switch I, which makes the nucleotide-binding pocket more solvent accessible, likely also facilitates exchange.
+
-
 
+
-
Insights regarding guanine nucleotide exchange from the structure of a DENN-domain protein complexed with its Rab GTPase substrate.,Wu X, Bradley MJ, Cai Y, Kummel D, De La Cruz EM, Barr FA, Reinisch KM Proc Natl Acad Sci U S A. 2011 Nov 7. PMID:22065758<ref>PMID:22065758</ref>
+
-
 
+
-
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
==See Also==
==See Also==
-
*[[GTP-binding protein|GTP-binding protein]]
+
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
 +
*[[Ras-related protein Rab 3D structures|Ras-related protein Rab 3D structures]]
== References ==
== References ==
<references/>
<references/>
Line 23: Line 18:
</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Kummel, D.]]
+
[[Category: Large Structures]]
-
[[Category: Reinisch, K M.]]
+
[[Category: Kummel D]]
-
[[Category: Wu, X D.]]
+
[[Category: Reinisch KM]]
-
[[Category: Guanine exchange factor]]
+
[[Category: Wu XD]]
-
[[Category: Longin domain]]
+
-
[[Category: Protein transport]]
+
-
[[Category: Rab gtpase]]
+

Current revision

GEF domain of DENND 1B in complex with Rab GTPase Rab35

PDB ID 3tw8

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3tw8"
Personal tools