3tup

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==Crystal structure of human mitochondrial PheRS complexed with tRNAPhe in the active open state==
==Crystal structure of human mitochondrial PheRS complexed with tRNAPhe in the active open state==
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<StructureSection load='3tup' size='340' side='right' caption='[[3tup]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
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<StructureSection load='3tup' size='340' side='right'caption='[[3tup]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3tup]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TUP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TUP FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3tup]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TUP FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3cmq|3cmq]], [[1pys|1pys]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.05&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FARS2, FARS1, HSPC320 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tup OCA], [https://pdbe.org/3tup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tup RCSB], [https://www.ebi.ac.uk/pdbsum/3tup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tup ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenylalanine--tRNA_ligase Phenylalanine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.20 6.1.1.20] </span></td></tr>
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</table>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tup OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tup RCSB], [http://www.ebi.ac.uk/pdbsum/3tup PDBsum]</span></td></tr>
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== Function ==
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<table>
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[https://www.uniprot.org/uniprot/SYFM_HUMAN SYFM_HUMAN] Catalyzes direct attachment of p-Tyr (Tyr) to tRNAPhe. Permits also, with a lower efficiency, the attachment of m-Tyr to tRNAPhe, thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins.<ref>PMID:19549855</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Monomeric human mitochondrial phenylalanyl-tRNA synthetase (PheRS), or hmPheRS, is the smallest known enzyme exhibiting aminoacylation activity. HmPheRS consists of only two structural domains and differs markedly from heterodimeric eukaryotic cytosolic and bacterial analogs both in the domain organization and in the mode of tRNA binding. Here, we describe the first crystal structure of mitochondrial aminoacyl-tRNA synthetase (aaRS) complexed with tRNA at a resolution of 3.0 A. Unlike bacterial PheRSs, the hmPheRS recognizes C74, the G1-C72 base pair, and the "discriminator" base A73, proposed to contribute to tRNA(Phe) identity in the yeast mitochondrial enzyme. An interaction of the tRNA acceptor stem with the signature motif 2 residues of hmPheRS is of critical importance for the stabilization of the CCA-extended conformation and its correct placement in the synthetic site of the enzyme. The crystal structure of hmPheRS-tRNA(Phe) provides direct evidence that the formation of the complex with tRNA requires a significant rearrangement of the anticodon-binding domain from the "closed" to the productive "open" state. Global repositioning of the domain is tRNA modulated and governed by long-range electrostatic interactions.
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Crystal Structure of Human Mitochondrial PheRS Complexed with tRNA(Phe) in the Active "Open" State.,Klipcan L, Moor N, Finarov I, Kessler N, Sukhanova M, Safro MG J Mol Biol. 2011 Nov 23. PMID:22137894<ref>PMID:22137894</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Phenylalanine--tRNA ligase]]
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[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Finarov, I.]]
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[[Category: Finarov I]]
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[[Category: Kessler, N.]]
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[[Category: Kessler N]]
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[[Category: Klipcan, L.]]
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[[Category: Klipcan L]]
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[[Category: Moor, N.]]
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[[Category: Moor N]]
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[[Category: Safro, M.]]
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[[Category: Safro M]]
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[[Category: Sukhanova, M.]]
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[[Category: Sukhanova M]]
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[[Category: Aminoacylation]]
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[[Category: Class ii aar]]
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[[Category: Ligase-rna complex]]
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[[Category: Mitochondria]]
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[[Category: Rrm fold]]
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Current revision

Crystal structure of human mitochondrial PheRS complexed with tRNAPhe in the active open state

PDB ID 3tup

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