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| | ==Crystal structure of a type 4 CDGSH iron-sulfur protein.== | | ==Crystal structure of a type 4 CDGSH iron-sulfur protein.== |
| - | <StructureSection load='3tbm' size='340' side='right' caption='[[3tbm]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='3tbm' size='340' side='right'caption='[[3tbm]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3tbm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TBM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TBM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3tbm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum_GMI1000 Ralstonia solanacearum GMI1000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TBM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TBM FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene><br> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.797Å</td></tr> |
| - | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tbn|3tbn]], [[3tbo|3tbo]], [[2qd0|2qd0]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr> |
| - | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RSc2912 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=305 Ralstonia solanacearum])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tbm OCA], [https://pdbe.org/3tbm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tbm RCSB], [https://www.ebi.ac.uk/pdbsum/3tbm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tbm ProSAT]</span></td></tr> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tbm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tbm RCSB], [http://www.ebi.ac.uk/pdbsum/3tbm PDBsum]</span></td></tr>
| + | </table> |
| - | <table> | + | == Function == |
| - | <div style="background-color:#fffaf0;">
| + | [https://www.uniprot.org/uniprot/Q8XVB9_RALSO Q8XVB9_RALSO] |
| - | == Publication Abstract from PubMed == | + | |
| - | The recently discovered CDGSH iron-sulfur domains (CISDs) are classified into seven major types with a wide distribution throughout the three domains of life. The type 1 protein mitoNEET has been shown to fold into a dimer with the signature CDGSH motif binding to a [2Fe-2S] cluster. However, the structures of all other types of CISDs were unknown. Here we report the crystal structures of type 3, 4, and 6 CISDs determined at 1.5 A, 1.8 A and 1.15 A resolution, respectively. The type 3 and 4 CISD each contain one CDGSH motif and adopt a dimeric structure. Although similar to each other, the two structures have permutated topologies, and both are distinct from the type 1 structure. The type 6 CISD contains tandem CDGSH motifs and adopts a monomeric structure with an internal pseudo dyad symmetry. All currently known CISD structures share dual iron-sulfur binding modules and a beta-sandwich for either intermolecular or intramolecular dimerization. The iron-sulfur binding module, the beta-strand N-terminal to the module and a proline motif are conserved among different type structures, but the dimerization module and the interface and orientation between the two iron-sulfur binding modules are divergent. Sequence analysis further shows resemblance between CISD types 4 and 7 and between 1 and 2. Our findings suggest that all CISDs share common ancestry and diverged into three primary folds with a characteristic phylogenetic distribution: a eukaryote-specific fold adopted by types 1 and 2 proteins, a prokaryote-specific fold adopted by types 3, 4 and 7 proteins, and a tandem-motif fold adopted by types 5 and 6 proteins. Our comprehensive structural, sequential and phylogenetic analysis provides significant insight into the assembly principles and evolutionary relationship of CISDs.
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| - | Structure and Molecular Evolution of CDGSH Iron-Sulfur Domains.,Lin J, Zhang L, Lai S, Ye K PLoS One. 2011;6(9):e24790. Epub 2011 Sep 16. PMID:21949752<ref>PMID:21949752</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ralstonia solanacearum]] | + | [[Category: Large Structures]] |
| - | [[Category: Lin, J.]] | + | [[Category: Ralstonia solanacearum GMI1000]] |
| - | [[Category: Ye, K.]] | + | [[Category: Lin J]] |
| - | [[Category: Zhang, L.]] | + | [[Category: Ye K]] |
| - | [[Category: Cdgsh]]
| + | [[Category: Zhang L]] |
| - | [[Category: Iron-sulfur]]
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| - | [[Category: Metal binding protein]]
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