4a0f
From Proteopedia
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==Structure of selenomethionine substituted bifunctional DAPA aminotransferase-dethiobiotin synthetase from Arabidopsis thaliana in its apo form.== | ==Structure of selenomethionine substituted bifunctional DAPA aminotransferase-dethiobiotin synthetase from Arabidopsis thaliana in its apo form.== | ||
| - | <StructureSection load='4a0f' size='340' side='right' caption='[[4a0f]], [[Resolution|resolution]] 2.71Å' scene=''> | + | <StructureSection load='4a0f' size='340' side='right'caption='[[4a0f]], [[Resolution|resolution]] 2.71Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[4a0f]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[4a0f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A0F FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>[[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.714Å</td></tr> |
| - | <tr><td class="sblockLbl"><b>[[ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | < | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a0f OCA], [https://pdbe.org/4a0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a0f RCSB], [https://www.ebi.ac.uk/pdbsum/4a0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a0f ProSAT]</span></td></tr> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </table> |
| - | <table> | + | == Function == |
| + | [https://www.uniprot.org/uniprot/BIODA_ARATH BIODA_ARATH] Bifunctional enzyme that catalyzes two different reactions involved in the biotin biosynthesis. Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring. Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only animotransferase known to utilize SAM as an amino donor. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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Biochemical and structural characterization of the Arabidopsis bifunctional enzyme dethiobiotin synthetase-diaminopelargonic Acid aminotransferase: evidence for substrate channeling in biotin synthesis.,Cobessi D, Dumas R, Pautre V, Meinguet C, Ferrer JL, Alban C Plant Cell. 2012 Apr;24(4):1608-25. Epub 2012 Apr 30. PMID:22547782<ref>PMID:22547782</ref> | Biochemical and structural characterization of the Arabidopsis bifunctional enzyme dethiobiotin synthetase-diaminopelargonic Acid aminotransferase: evidence for substrate channeling in biotin synthesis.,Cobessi D, Dumas R, Pautre V, Meinguet C, Ferrer JL, Alban C Plant Cell. 2012 Apr;24(4):1608-25. Epub 2012 Apr 30. PMID:22547782<ref>PMID:22547782</ref> | ||
| - | From | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
</div> | </div> | ||
| + | <div class="pdbe-citations 4a0f" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[7%2C8-diaminopelargonic acid synthase 3D structures|7%2C8-diaminopelargonic acid synthase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
| - | [[Category: Alban | + | [[Category: Large Structures]] |
| - | [[Category: Cobessi | + | [[Category: Alban C]] |
| - | [[Category: Dumas | + | [[Category: Cobessi D]] |
| - | [[Category: Ferrer | + | [[Category: Dumas R]] |
| - | [[Category: Meinguet | + | [[Category: Ferrer JL]] |
| - | [[Category: Pautre | + | [[Category: Meinguet C]] |
| - | + | [[Category: Pautre V]] | |
| - | + | ||
| - | + | ||
Current revision
Structure of selenomethionine substituted bifunctional DAPA aminotransferase-dethiobiotin synthetase from Arabidopsis thaliana in its apo form.
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