This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1b73

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (23:20, 27 December 2023) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1b73.jpg|left|200px]]
 
-
{{Structure
+
==GLUTAMATE RACEMASE FROM AQUIFEX PYROPHILUS==
-
|PDB= 1b73 |SIZE=350|CAPTION= <scene name='initialview01'>1b73</scene>, resolution 2.3&Aring;
+
<StructureSection load='1b73' size='340' side='right'caption='[[1b73]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[1b73]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_pyrophilus Aquifex pyrophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B73 FirstGlance]. <br>
-
|ACTIVITY= [http://en.wikipedia.org/wiki/Glutamate_racemase Glutamate racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.3 5.1.1.3]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b73 OCA], [https://pdbe.org/1b73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b73 RCSB], [https://www.ebi.ac.uk/pdbsum/1b73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b73 ProSAT]</span></td></tr>
-
}}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MURI_AQUPY MURI_AQUPY] Provides the (R)-glutamate required for cell wall biosynthesis. Converts L- or D-glutamate to D- or L-glutamate, respectively, but not other amino acids such as alanine, aspartate, and glutamine.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b7/1b73_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b73 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Glutamate racemase (MurI) is responsible for the synthesis of D-glutamate, an essential building block of the peptidoglycan layer in bacterial cell walls. The crystal structure of glutamate racemase from Aquifex pyrophilus, determined at 2.3 A resolution, reveals that the enzyme forms a dimer and each monomer consists of two alpha/beta fold domains, a unique structure that has not been observed in other racemases or members of an enolase superfamily. A substrate analog, D-glutamine, binds to the deep pocket formed by conserved residues from two monomers. The structural and mutational analyses allow us to propose a mechanism of metal cofactor-independent glutamate racemase in which two cysteine residues are involved in catalysis.
-
'''GLUTAMATE RACEMASE FROM AQUIFEX PYROPHILUS'''
+
Structure and mechanism of glutamate racemase from Aquifex pyrophilus.,Hwang KY, Cho CS, Kim SS, Sung HC, Yu YG, Cho Y Nat Struct Biol. 1999 May;6(5):422-6. PMID:10331867<ref>PMID:10331867</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1b73" style="background-color:#fffaf0;"></div>
-
==Overview==
+
==See Also==
-
Glutamate racemase (MurI) is responsible for the synthesis of D-glutamate, an essential building block of the peptidoglycan layer in bacterial cell walls. The crystal structure of glutamate racemase from Aquifex pyrophilus, determined at 2.3 A resolution, reveals that the enzyme forms a dimer and each monomer consists of two alpha/beta fold domains, a unique structure that has not been observed in other racemases or members of an enolase superfamily. A substrate analog, D-glutamine, binds to the deep pocket formed by conserved residues from two monomers. The structural and mutational analyses allow us to propose a mechanism of metal cofactor-independent glutamate racemase in which two cysteine residues are involved in catalysis.
+
*[[Glutamate racemase 3D structures|Glutamate racemase 3D structures]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
1B73 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_pyrophilus Aquifex pyrophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B73 OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
-
Structure and mechanism of glutamate racemase from Aquifex pyrophilus., Hwang KY, Cho CS, Kim SS, Sung HC, Yu YG, Cho Y, Nat Struct Biol. 1999 May;6(5):422-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10331867 10331867]
+
[[Category: Aquifex pyrophilus]]
[[Category: Aquifex pyrophilus]]
-
[[Category: Glutamate racemase]]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Cho CS]]
-
[[Category: Cho, C S.]]
+
[[Category: Cho Y]]
-
[[Category: Cho, Y.]]
+
[[Category: Hwang KY]]
-
[[Category: Hwang, K Y.]]
+
[[Category: Kim SS]]
-
[[Category: Kim, S S.]]
+
[[Category: Yu YG]]
-
[[Category: Yu, Y G.]]
+
-
[[Category: isomerase]]
+
-
[[Category: racemase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:07:09 2008''
+

Current revision

GLUTAMATE RACEMASE FROM AQUIFEX PYROPHILUS

PDB ID 1b73

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1b73"
Personal tools