4dzt

<StructureSection load='4dzt' size='340' side='right' caption='[[4dzt]], [[Resolution|resolution]] 1.95&Aring;' scene=''>

<table><tr><td colspan='2'>[[4dzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DZT FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene><br>

<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2prk|2prk]], [[1sup|1sup]], [[2gko|2gko]], [[1svn|1svn]]</td></tr>

<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aqualysin_1 Aqualysin 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.111 3.4.21.111] </span></td></tr>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dzt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dzt RCSB], [http://www.ebi.ac.uk/pdbsum/4dzt PDBsum]</span></td></tr>

<table>

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== Publication Abstract from PubMed ==

Aqualysin I, a thermostable protease found in the culture medium of Thermus aquaticus YT-1, has been purified to homogeneity using a combination of ion-exchange and affinity chromatography. It is a polypeptide with a molecular weight of 28 350 [Kwon, Terada, Matsuzawa &amp; Ohta (1988). Eur. J. Biochem. 173, 491-497] and is most active at 343-353 K and pH about 10.0 [Matsuzawa, Tokugawa, Hamaoki, Mizoguchi, Taguchi, Terada, Kwon &amp; Ohta (1988). Eur. J. Biochem. 171, 441-447]. Crystals of the enzyme are monoclinic, space group P2(1), with cell dimensions a = 40.80 (5), b = 64.39 (6), c = 45.51 (6) A and beta = 109.1 (1) degrees. The asymmetric unit consists of a single molecule (V(m) = 1.99 A(3)Da(-1)). The crystals are stable to X-radiation and scatter to at least 2.8 A resolution.

 

Purification, crystallization and preliminary X-ray investigation of aqualysin I, a heat-stable serine protease.,Green PR, Oliver JD, Strickland LC, Toerner DR, Matsuzawa H, Ohta T Acta Crystallogr D Biol Crystallogr. 1993 May 1;49(Pt 3):349-52. PMID:15299524<ref>PMID:15299524</ref>

 

[[Category: Aqualysin 1]]

[[Category: Barnett, B L.]]

[[Category: Green, P R.]]

[[Category: Oliver, J D.]]

[[Category: Rydel, T.]]

[[Category: Strickland, L C.]]

[[Category: Sullivan, J F.]]

[[Category: Serine protease]]