483d

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==CRYSTAL STRUCTURE OF THE SARCIN/RICIN DOMAIN FROM E. COLI 23 S RRNA==
==CRYSTAL STRUCTURE OF THE SARCIN/RICIN DOMAIN FROM E. COLI 23 S RRNA==
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<StructureSection load='483d' size='340' side='right' caption='[[483d]], [[Resolution|resolution]] 1.11&Aring;' scene=''>
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<StructureSection load='483d' size='340' side='right'caption='[[483d]], [[Resolution|resolution]] 1.11&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[483d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=483D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=483D FirstGlance]. <br>
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<table><tr><td colspan='2'>[[483d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=483D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=483D FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=483d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=483d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=483d RCSB], [http://www.ebi.ac.uk/pdbsum/483d PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.11&#8491;</td></tr>
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<table>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=483d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=483d OCA], [https://pdbe.org/483d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=483d RCSB], [https://www.ebi.ac.uk/pdbsum/483d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=483d ProSAT]</span></td></tr>
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<div style="background-color:#fffaf0;">
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</table>
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== Publication Abstract from PubMed ==
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The sarcin/ricin domain of 23 S - 28 S ribosomal RNA is essential for protein synthesis because it forms a critical part of the binding site for elongation factors. A crystal structure of an RNA of 27 nucleotides that mimics the domain in Escherichia coli 23 S rRNA was determined at 1.11 A resolution. The domain folds into a hairpin distorted by four non-canonical base-pairs and one base triple. The fold is stabilized by cross-strand and intra-stand stacking; no intramolecular stabilizing metal ions are observed. This is the first structure to reveal in great detail the geometry and the hydration of two common motifs that are conserved in this rRNA domain, a GAGA tetraloop and a G-bulged cross-strand A stack. Differences in the region connecting these motifs to the stem in the E. coli and in the rat sarcin/ricin domains may contribute to the species-specific binding of elongation factors. Correlation of nucleotide protection data with the structure indicates that the domain has two surfaces. One surface is accessible, lies primarily in the major groove, and is likely to bind the elongation factors. The second lies primarily in the minor groove, and is likely to be buried in the ribosome. This minor groove surface includes the Watson-Crick faces of the cytosine bases in the unusual A2654.C2666 and U2653.C2667 water-mediated base-pairs.
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The two faces of the Escherichia coli 23 S rRNA sarcin/ricin domain: the structure at 1.11 A resolution.,Correll CC, Wool IG, Munishkin A J Mol Biol. 1999 Sep 17;292(2):275-87. PMID:10493875<ref>PMID:10493875</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Correll, C C.]]
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[[Category: Large Structures]]
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[[Category: Munishkin, A.]]
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[[Category: Correll CC]]
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[[Category: Wool, I G.]]
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[[Category: Munishkin A]]
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[[Category: Rna]]
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[[Category: Wool IG]]
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[[Category: Sacin/ricin domain rna recognition ribosomes elongation factors crystal structure]]
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Current revision

CRYSTAL STRUCTURE OF THE SARCIN/RICIN DOMAIN FROM E. COLI 23 S RRNA

PDB ID 483d

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