1mlx

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:45, 14 February 2024) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex==
==Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex==
-
<StructureSection load='1mlx' size='340' side='right' caption='[[1mlx]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
+
<StructureSection load='1mlx' size='340' side='right'caption='[[1mlx]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLX FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MLX FirstGlance]. <br>
-
</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
-
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [http://www.ebi.ac.uk/pdbsum/1mlx PDBsum]</span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>
-
<table>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [https://pdbe.org/1mlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [https://www.ebi.ac.uk/pdbsum/1mlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlx ProSAT]</span></td></tr>
-
<div style="background-color:#fffaf0;">
+
</table>
-
== Publication Abstract from PubMed ==
+
-
A novel 2'-modification, 2'-O-[2-(methylthio)ethyl] or 2'-O-MTE, has been incorporated into oligonucleotides and evaluated for properties relevant to antisense activity. The results were compared with the previously characterized 2'-O-[2-(methoxy)ethyl] 2'-O-MOE modification. As expected, the 2'-O-MTE modified oligonucleotides exhibited improved binding to human serum albumin compared to the 2'-O-MOE modified oligonucleotides. The 2'-O-MTE oligonucleotides maintained high binding affinity to target RNA. Nuclease digestion of 2'-O-MTE oligonucleotides showed that they have limited resistance to exonuclease degradation. We analyzed the crystal structure of a decamer DNA duplex containing the 2'-O-MTE modifcation. Analysis of the crystal structure provides insight into the improved RNA binding affinity, protein binding affinity and limited resistance of 2'-O-MTE modified oligonucleotides to exonuclease degradation.
+
-
 
+
-
2'-O-[2-(methylthio)ethyl]-modified oligonucleotide: an analogue of 2'-O-[2-(methoxy)-ethyl]-modified oligonucleotide with improved protein binding properties and high binding affinity to target RNA.,Prakash TP, Manoharan M, Kawasaki AM, Fraser AS, Lesnik EA, Sioufi N, Leeds JM, Teplova M, Egli M Biochemistry. 2002 Oct 1;41(39):11642-8. PMID:12269806<ref>PMID:12269806</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Egli, M.]]
+
[[Category: Large Structures]]
-
[[Category: Fraser, A S.]]
+
[[Category: Egli M]]
-
[[Category: Kawasaki, A M.]]
+
[[Category: Fraser AS]]
-
[[Category: Leeds, J M.]]
+
[[Category: Kawasaki AM]]
-
[[Category: Lesnik, E A.]]
+
[[Category: Leeds JM]]
-
[[Category: Manoharan, M.]]
+
[[Category: Lesnik EA]]
-
[[Category: Prakash, T P.]]
+
[[Category: Manoharan M]]
-
[[Category: Sioufi, N.]]
+
[[Category: Prakash TP]]
-
[[Category: Teplova, M.]]
+
[[Category: Sioufi N]]
-
[[Category: A-form]]
+
[[Category: Teplova M]]
-
[[Category: Antisense modification]]
+
-
[[Category: Dna]]
+
-
[[Category: Double helix]]
+
-
[[Category: High resolution structure]]
+
-
[[Category: Hydration]]
+
-
[[Category: Nuclease resistance]]
+
-
[[Category: Nucleic acid analogue]]
+
-
[[Category: Protein binding affinity]]
+
-
[[Category: Rna affinity]]
+

Current revision

Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex

PDB ID 1mlx

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mlx"
Personal tools