|
|
| (6 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | ==PHEROMONE ER-23 FROM EUPLOTES RAIKOVI== | + | |
| - | <StructureSection load='1ha8' size='340' side='right' caption='[[1ha8]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | ==Pheromone Er-23 from Euplotes raikovi== |
| | + | <StructureSection load='1ha8' size='340' side='right'caption='[[1ha8]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ha8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Euplotes_raikovi Euplotes raikovi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HA8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HA8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ha8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Euplotes_raikovi Euplotes raikovi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HA8 FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ha8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ha8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ha8 RCSB], [http://www.ebi.ac.uk/pdbsum/1ha8 PDBsum]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <table> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ha8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ha8 OCA], [https://pdbe.org/1ha8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ha8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ha8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ha8 ProSAT]</span></td></tr> |
| | + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/MER23_EUPRA MER23_EUPRA] Mating ciliate pheromones (or gamones) are diffusible extracellular communication signals that distinguish different intraspecific classes of cells commonly referred to as 'mating types'. They prepare the latter for conjugation by changing their cell surface properties. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 13: |
Line 17: |
| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 1ha8" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| Line 18: |
Line 23: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Euplotes raikovi]] | | [[Category: Euplotes raikovi]] |
| - | [[Category: Damberger, F.]] | + | [[Category: Large Structures]] |
| - | [[Category: Luporini, P.]] | + | [[Category: Damberger F]] |
| - | [[Category: Wuthrich, K.]] | + | [[Category: Luporini P]] |
| - | [[Category: Zahn, R.]] | + | [[Category: Wuthrich K]] |
| - | [[Category: Pheromone]]
| + | [[Category: Zahn R]] |
| Structural highlights
Function
MER23_EUPRA Mating ciliate pheromones (or gamones) are diffusible extracellular communication signals that distinguish different intraspecific classes of cells commonly referred to as 'mating types'. They prepare the latter for conjugation by changing their cell surface properties.
Publication Abstract from PubMed
The NMR solution structure of the 51 residue pheromone Er-23 from the ciliated protozoan Euplotes raikovi (Er) was calculated with the torsion angle dynamics program DYANA from 582 nuclear Overhauser enhancement (NOE) upper limit distance constraints, 46 dihedral angle constraints and 30 disulfide bond constraints. The disulfide bridges had not been assigned by chemical methods, and initially were assigned tentatively on the basis of inspection of the positioning of the Cys sulfhydryl groups in a bundle of 20 conformers that was calculated without disulfide bond constraints. The assignment of disulfide bridges was then validated by structure calculations that assessed the compatibility of plausible alternative Cys-Cys disulfide combinations with the input of NOE upper distance constraints and dihedral angle constraints. For a group of 20 conformers used to characterize the solution structure, the average pairwise root-mean-square distances from the mean coordinates calculated for the backbone heavy atoms N, C(alpha) and C' of resideus 1-51 is 0.38 A. The molecular architecture consists of a three-dimensional arrangement of five helices comprised of residues 2-8, 14-17, 26-29, 34-36 and 38-47, with five disulfide bridges in the positions 3-24, 6-16, 13-47, 27-40, and 35-51, which has so far not been represented in the Protein Data Bank. Er-23 is unique among presently known Er-pheromones with respect to size, sequence, the number of disulfide bonds and the three-dimensional structure, thus providing a new structural basis for rationalizing the physiological functions of this protein family.
NMR structure of the Euplotes raikovi pheromone Er-23 and identification of its five disulfide bonds.,Zahn R, Damberger F, Ortenzi C, Luporini P, Wuthrich K J Mol Biol. 2001 Nov 9;313(5):923-31. PMID:11700049[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zahn R, Damberger F, Ortenzi C, Luporini P, Wuthrich K. NMR structure of the Euplotes raikovi pheromone Er-23 and identification of its five disulfide bonds. J Mol Biol. 2001 Nov 9;313(5):923-31. PMID:11700049 doi:10.1006/jmbi.2001.5099
|
