1bn8

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[[Image:1bn8.jpg|left|200px]]
 
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{{Structure
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==BACILLUS SUBTILIS PECTATE LYASE==
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|PDB= 1bn8 |SIZE=350|CAPTION= <scene name='initialview01'>1bn8</scene>, resolution 1.8&Aring;
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<StructureSection load='1bn8' size='340' side='right'caption='[[1bn8]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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<table><tr><td colspan='2'>[[1bn8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BN8 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Pectate_lyase Pectate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.2 4.2.2.2]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bn8 OCA], [https://pdbe.org/1bn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bn8 RCSB], [https://www.ebi.ac.uk/pdbsum/1bn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bn8 ProSAT]</span></td></tr>
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</table>
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'''BACILLUS SUBTILIS PECTATE LYASE'''
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== Function ==
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[https://www.uniprot.org/uniprot/PLY_BACSU PLY_BACSU] Produces unsaturated products from polygalacturonate.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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We have solved the structure of the Bacillus subtilis pectate lyase (BsPel) in complex with calcium. The structure consists of a parallel beta-helix domain and a loop region. The alpha L-bounded beta-strand seen in BsPel is a new element of protein structure and its frequent occurrence suggests it is an important characteristic of the parallel beta-helix. A pronounced cleft is formed between the loops and the parallel beta-helix domain and we propose that this is the active site cleft. Calcium, essential for the activity of the enzyme, binds at the bottom of this cleft and an arginine residue close to the calcium, which is conserved across all pectin and pectate lyases, may be involved in catalysis.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bn/1bn8_consurf.spt"</scriptWhenChecked>
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1BN8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BN8 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The structure of Bacillus subtilis pectate lyase in complex with calcium., Pickersgill R, Jenkins J, Harris G, Nasser W, Robert-Baudouy J, Nat Struct Biol. 1994 Oct;1(10):717-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7634076 7634076]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bn8 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Pectate lyase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Harris G]]
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[[Category: Harris, G.]]
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[[Category: Jenkins J]]
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[[Category: Jenkins, J.]]
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[[Category: Pickersgill R]]
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[[Category: Pickersgill, R.]]
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[[Category: CA]]
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[[Category: lyase]]
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[[Category: parallel beta-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:13:16 2008''
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Current revision

BACILLUS SUBTILIS PECTATE LYASE

PDB ID 1bn8

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