1bos

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[[Image:1bos.gif|left|200px]]
 
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{{Structure
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==SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR==
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|PDB= 1bos |SIZE=350|CAPTION= <scene name='initialview01'>1bos</scene>, resolution 2.8&Aring;
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<StructureSection load='1bos' size='340' side='right'caption='[[1bos]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>
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<table><tr><td colspan='2'>[[1bos]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_H-19B Enterobacteria phage H-19B] and [https://en.wikipedia.org/wiki/Phage_h30 Phage h30]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BOS FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bos OCA], [https://pdbe.org/1bos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bos RCSB], [https://www.ebi.ac.uk/pdbsum/1bos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bos ProSAT]</span></td></tr>
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</table>
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'''SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR'''
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== Function ==
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[https://www.uniprot.org/uniprot/STXB_BPH19 STXB_BPH19] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/1bos_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bos ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells.
Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells.
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==About this Structure==
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Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3.,Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:9485303<ref>PMID:9485303</ref>
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1BOS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_h30,_bacteriophage_h19b Bacteriophage h30, bacteriophage h19b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3., Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ, Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9485303 9485303]
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</div>
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[[Category: Bacteriophage h30, bacteriophage h19b]]
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<div class="pdbe-citations 1bos" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Armstrong, G D.]]
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[[Category: Boodhoo, A.]]
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[[Category: Brunton, J L.]]
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[[Category: Cummings, M D.]]
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[[Category: Hazes, B.]]
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[[Category: Ling, H.]]
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[[Category: Read, R J.]]
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[[Category: GAL]]
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[[Category: ob-fold]]
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[[Category: protein-carbohydrate recognition]]
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[[Category: receptor binding]]
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[[Category: toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:13:50 2008''
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==See Also==
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*[[Shiga toxin 3D structures|Shiga toxin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Enterobacteria phage H-19B]]
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[[Category: Large Structures]]
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[[Category: Phage h30]]
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[[Category: Armstrong GD]]
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[[Category: Boodhoo A]]
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[[Category: Brunton JL]]
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[[Category: Cummings MD]]
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[[Category: Hazes B]]
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[[Category: Ling H]]
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[[Category: Read RJ]]

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SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR

PDB ID 1bos

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