4pne
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of the [4+2]-Cyclase SpnF== | |
| + | <StructureSection load='4pne' size='340' side='right'caption='[[4pne]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4pne]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharopolyspora_spinosa Saccharopolyspora spinosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PNE FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pne OCA], [https://pdbe.org/4pne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pne RCSB], [https://www.ebi.ac.uk/pdbsum/4pne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pne ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9ALM7_SACSN Q9ALM7_SACSN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In the biosynthetic pathway of the spinosyn insecticides, the tailoring enzyme SpnF performs a [4 + 2] cycloaddition on a 22-membered macrolactone to forge an embedded cyclohexene ring. To learn more about this reaction, which could potentially proceed through a Diels-Alder mechanism, we determined the 1.50-A-resolution crystal structure of SpnF bound to S-adenosylhomocysteine. This sets the stage for advanced experimental and computational studies to determine the precise mechanism of SpnF-mediated cyclization. | ||
| - | + | The structure of SpnF, a standalone enzyme that catalyzes [4 + 2] cycloaddition.,Fage CD, Isiorho EA, Liu Y, Wagner DT, Liu HW, Keatinge-Clay AT Nat Chem Biol. 2015 Mar 2. doi: 10.1038/nchembio.1768. PMID:25730549<ref>PMID:25730549</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4pne" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharopolyspora spinosa]] | ||
| + | [[Category: Fage CD]] | ||
| + | [[Category: Isiorho EA]] | ||
| + | [[Category: Keatinge-Clay AT]] | ||
| + | [[Category: Liu H-W]] | ||
| + | [[Category: Liu Y-N]] | ||
Current revision
Crystal Structure of the [4+2]-Cyclase SpnF
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