4uok
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Electron Cryo-microscopy of Venezuelan Equine Encephalitis Virus TC-83 in complex with neutralizing antibody Fab 3B4C-4== | |
| + | <SX load='4uok' size='340' side='right' viewer='molstar' caption='[[4uok]], [[Resolution|resolution]] 18.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4uok]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UOK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UOK FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uok OCA], [https://pdbe.org/4uok PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uok RCSB], [https://www.ebi.ac.uk/pdbsum/4uok PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uok ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Alphaviruses are serious, sometimes lethal human pathogens that belong to the family Togaviridae. The structures of human Venezuelan equine encephalitis virus (VEEV), an alphavirus, in complex with two strongly neutralizing antibody Fab fragments (F5 and 3B4C-4) have been determined using a combination of cryo-electron microscopy and homology modeling. We characterize these monoclonal antibody Fab fragments, which are known to abrogate VEEV infectivity by binding to the E2 (envelope) surface glycoprotein. Both of these antibody Fab fragments cross-link the surface E2 glycoproteins and therefore probably inhibit infectivity by blocking the conformational changes that are required for making the virus fusogenic. The F5 Fab fragment cross-links E2 proteins within one trimeric spike, whereas the 3B4C-4 Fab fragment cross-links E2 proteins from neighboring spikes. Furthermore, F5 probably blocks the receptor-binding site, whereas 3B4C-4 sterically hinders the exposure of the fusion loop at the end of the E2 B-domain. IMPORTANCE: Alphaviral infections are transmitted mainly by mosquitoes. Venezuelan equine encephalitis virus (VEEV) is an alphavirus with a wide distribution across the globe. No effective vaccines exist for alphaviral infections. Therefore, a better understanding of VEEV and its associated neutralizing antibodies will help with the development of effective drugs and vaccines. | ||
| - | + | Locking and blocking the viral landscape of an alphavirus with neutralizing antibodies.,Porta J, Jose J, Roehrig JT, Blair CD, Kuhn RJ, Rossmann MG J Virol. 2014 Sep 1;88(17):9616-23. doi: 10.1128/JVI.01286-14. Epub 2014 Jun 11. PMID:24920796<ref>PMID:24920796</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4uok" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Blair CD]] | ||
| + | [[Category: Jose J]] | ||
| + | [[Category: Kuhn RJ]] | ||
| + | [[Category: Porta J]] | ||
| + | [[Category: Roehrig JT]] | ||
| + | [[Category: Rossmann MG]] | ||
Current revision
Electron Cryo-microscopy of Venezuelan Equine Encephalitis Virus TC-83 in complex with neutralizing antibody Fab 3B4C-4
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Categories: Homo sapiens | Large Structures | Blair CD | Jose J | Kuhn RJ | Porta J | Roehrig JT | Rossmann MG
