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2cbf

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[[Image:2cbf.gif|left|200px]]<br />
 
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<applet load="2cbf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cbf, resolution 3.1&Aring;" />
 
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'''THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF'''<br />
 
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==Overview==
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==THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF==
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Biosynthesis of the corrin ring of vitamin B12 requires the action of six, S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely, related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been, determined to a resolution of 2.4 A. CbiF contains two alphabeta domains, forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The, location of AdoHcy and a number of conserved residues, helps define the, precorrin binding site. A second crystal form determined at 3.1 A, resolution highlights the flexibility of two loops around this site. CbiF, employs a unique mode of AdoHcy binding and represents a new class of, transmethylase.
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<StructureSection load='2cbf' size='340' side='right'caption='[[2cbf]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CBF FirstGlance]. <br>
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2CBF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]] with SAH as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133]]. Structure known Active Sites: FLP and SAH. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [https://pdbe.org/2cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [https://www.ebi.ac.uk/pdbsum/2cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cbf ProSAT]</span></td></tr>
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The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase., Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ, Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9665173 9665173]
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</table>
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[[Category: Bacillus megaterium]]
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== Function ==
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[[Category: Precorrin-4 C(11)-methyltransferase]]
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[https://www.uniprot.org/uniprot/CBIF_PRIMG CBIF_PRIMG] Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.<ref>PMID:23922391</ref>
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Raux, E.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Schubert, H.L.]]
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Check<jmol>
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[[Category: Warren, M.J.]]
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<jmolCheckbox>
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[[Category: Wilson, K.S.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/2cbf_consurf.spt"</scriptWhenChecked>
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[[Category: Woodcock, S.C.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: SAH]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: cobalamin biosynthesis]]
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</jmolCheckbox>
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[[Category: methylase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cbf ConSurf].
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[[Category: precorrin-4 methyltransferase]]
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<div style="clear:both"></div>
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== References ==
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 17:03:48 2007''
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Priestia megaterium]]
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[[Category: Raux E]]
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[[Category: Schubert HL]]
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[[Category: Warren MJ]]
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[[Category: Wilson KS]]
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[[Category: Woodcock SC]]

Current revision

THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF

PDB ID 2cbf

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