4f8g

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==Three-dimensional DNA lattices with non-canonical base pairs==
==Three-dimensional DNA lattices with non-canonical base pairs==
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<StructureSection load='4f8g' size='340' side='right' caption='[[4f8g]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
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<StructureSection load='4f8g' size='340' side='right'caption='[[4f8g]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4f8g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F8G FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4f8g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F8G FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4f8i|4f8i]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f8g RCSB], [http://www.ebi.ac.uk/pdbsum/4f8g PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8g OCA], [https://pdbe.org/4f8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f8g RCSB], [https://www.ebi.ac.uk/pdbsum/4f8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f8g ProSAT]</span></td></tr>
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<table>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Three-dimensional (3D) DNA crystals have been envisioned as programmable biomaterial scaffolds for creating ordered arrays of biological and nonbiological molecules. Despite having excellent programmable properties, the linearity of the Watson-Crick B-form duplex imposes limitations on 3D crystal design. Predictable noncanonical base pairing motifs have the potential to serve as junctions to connect linear DNA segments into complex 3D lattices. Here, we designed crystals based on a template structure with parallel-stranded noncanonical base pairs. Depending on pH, the structures we determined contained all but one or two of the designed secondary structure interactions. Surprisingly, a conformational change of the designed Watson-Crick duplex region resulted in crystal packing differences between the predicted and observed structures. However, the designed noncanonical motif was virtually identical to the template when crystals were grown at pH 5.5, highlighting the motif's predictability. At pH 7.0 we observed a structurally similar variation on this motif that contains a previously unobserved C-G*G-C quadruple base pair. We demonstrate that these two variants can interconvert in crystallo in response to pH perturbations. This study spotlights several important considerations in DNA crystal design, describes the first 3D DNA lattice composed of A-DNA helical sheets, and reveals a noncanonical DNA motif that has adaptive features that may be useful for designing dynamic crystals or biomaterial assemblies.
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Three-Dimensional DNA Crystals with pH-Responsive Noncanonical Junctions.,Muser SE, Paukstelis PJ J Am Chem Soc. 2012 Jul 18. PMID:22768973<ref>PMID:22768973</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Muser, S E.]]
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[[Category: Large Structures]]
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[[Category: Paukstelis, P J.]]
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[[Category: Muser SE]]
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[[Category: Crystal design]]
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[[Category: Paukstelis PJ]]
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[[Category: Dna]]
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[[Category: Dna nanotechnology]]
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[[Category: Non-canonical dna]]
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[[Category: Parallel dna]]
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Current revision

Three-dimensional DNA lattices with non-canonical base pairs

PDB ID 4f8g

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