4ghm

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==Crystal Structure of the H233A mutant of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with preQ0==
==Crystal Structure of the H233A mutant of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with preQ0==
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<StructureSection load='4ghm' size='340' side='right' caption='[[4ghm]], [[Resolution|resolution]] 1.62&Aring;' scene=''>
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<StructureSection load='4ghm' size='340' side='right'caption='[[4ghm]], [[Resolution|resolution]] 1.62&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4ghm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae_o1_biovar_el_tor Vibrio cholerae o1 biovar el tor]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3rzq 3rzq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GHM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GHM FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4ghm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3rzq 3rzq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GHM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GHM FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRF:7-DEAZA-7-AMINOMETHYL-GUANINE'>PRF</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.618&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PRF:7-DEAZA-7-AMINOMETHYL-GUANINE'>PRF</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bp1|3bp1]], [[3rjb|3rjb]], [[3rj4|3rj4]], [[3rzp|3rzp]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ghm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ghm OCA], [https://pdbe.org/4ghm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ghm RCSB], [https://www.ebi.ac.uk/pdbsum/4ghm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ghm ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">queF, VC_0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=686 Vibrio cholerae O1 biovar El Tor])</td></tr>
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</table>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/PreQ(1)_synthase PreQ(1) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.1.13 1.7.1.13] </span></td></tr>
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== Function ==
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ghm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ghm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ghm RCSB], [http://www.ebi.ac.uk/pdbsum/4ghm PDBsum]</span></td></tr>
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[https://www.uniprot.org/uniprot/QUEF_VIBCH QUEF_VIBCH] Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
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<table>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Vibrio cholerae o1 biovar el tor]]
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[[Category: Large Structures]]
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[[Category: Anderson, W F.]]
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[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
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[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]
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[[Category: Anderson WF]]
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[[Category: Gu, M.]]
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[[Category: Gu M]]
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[[Category: Joachimiak, A.]]
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[[Category: Joachimiak A]]
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[[Category: Kim, Y.]]
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[[Category: Kim Y]]
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[[Category: Zhou, M.]]
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[[Category: Zhou M]]
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[[Category: Alpha-beta structure]]
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[[Category: Center for structural genomics of infectious disease]]
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[[Category: Csgid]]
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[[Category: National institute of allergy and infectious disease]]
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[[Category: Niaid]]
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[[Category: Oxidoreductase]]
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[[Category: Structural genomic]]
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[[Category: Tunneling fold]]
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Current revision

Crystal Structure of the H233A mutant of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with preQ0

PDB ID 4ghm

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