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4aqx

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==Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of magnesium==
==Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of magnesium==
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<StructureSection load='4aqx' size='340' side='right' caption='[[4aqx]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<StructureSection load='4aqx' size='340' side='right'caption='[[4aqx]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4aqx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AQX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4aqx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AQX FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1af5|1af5]], [[1bp7|1bp7]], [[1g9y|1g9y]], [[1g9z|1g9z]], [[1mow|1mow]], [[1n3e|1n3e]], [[1n3f|1n3f]], [[1t9i|1t9i]], [[1t9j|1t9j]], [[1u0c|1u0c]], [[1u0d|1u0d]], [[2vbj|2vbj]], [[2vbl|2vbl]], [[2vbn|2vbn]], [[2vbo|2vbo]], [[4aab|4aab]], [[4aad|4aad]], [[4aae|4aae]], [[4aaf|4aaf]], [[4aag|4aag]], [[4aqu|4aqu]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqx OCA], [https://pdbe.org/4aqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aqx RCSB], [https://www.ebi.ac.uk/pdbsum/4aqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aqx ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aqx RCSB], [http://www.ebi.ac.uk/pdbsum/4aqx PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
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<div class="pdbe-citations 4aqx" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
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*[[Endonuclease|Endonuclease]]
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Chlamydomonas reinhardtii]]
[[Category: Chlamydomonas reinhardtii]]
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[[Category: Daboussi, F.]]
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[[Category: Large Structures]]
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[[Category: Duchateau, P.]]
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[[Category: Synthetic construct]]
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[[Category: Leduc, S.]]
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[[Category: Daboussi F]]
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[[Category: Macmaster, R.]]
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[[Category: Duchateau P]]
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[[Category: Molina, R.]]
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[[Category: Leduc S]]
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[[Category: Montoya, G.]]
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[[Category: Macmaster R]]
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[[Category: Redondo, P.]]
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[[Category: Molina R]]
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[[Category: Valton, J.]]
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[[Category: Montoya G]]
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[[Category: Gene targeting]]
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[[Category: Redondo P]]
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[[Category: Genetic]]
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[[Category: Valton J]]
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[[Category: Homing endonuclease]]
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[[Category: Hydrolase]]
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[[Category: Methylation]]
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[[Category: Protein-dna interaction]]
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Current revision

Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of magnesium

PDB ID 4aqx

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