1byl

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[[Image:1byl.jpg|left|200px]]
 
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{{Structure
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==BLEOMYCIN RESISTANCE PROTEIN FROM STREPTOALLOTEICHUS HINDUSTANUS==
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|PDB= 1byl |SIZE=350|CAPTION= <scene name='initialview01'>1byl</scene>, resolution 2.3&Aring;
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<StructureSection load='1byl' size='340' side='right'caption='[[1byl]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1byl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptoalloteichus_hindustanus Streptoalloteichus hindustanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BYL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BYL FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE= SH BLE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2017 Streptoalloteichus hindustanus])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1byl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1byl OCA], [https://pdbe.org/1byl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1byl RCSB], [https://www.ebi.ac.uk/pdbsum/1byl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1byl ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''BLEOMYCIN RESISTANCE PROTEIN FROM STREPTOALLOTEICHUS HINDUSTANUS'''
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[https://www.uniprot.org/uniprot/BLE_STRHI BLE_STRHI] Binding protein with a strong affinity to the bleomycin family of antibiotics.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The antibiotic bleomycin, a strong DNA cutting agent, is naturally produced by actinomycetes which have developed a resistance mechanism against such a lethal compound. The crystal structure, at 2.3 A resolution, of a bleomycin resistance protein of 14 kDa reveals a structure in two halves with the same alpha/beta fold despite no sequence similarity. The crystal packing shows compact dimers with a hydrophobic interface and involved in mutual chain exchange. Two independent solution studies (analytical centrifugation and light scattering) showed that this dimeric form is not a packing artefact but is indeed the functional one. Furthermore, light scattering also showed that one dimer binds two antibiotic molecules as expected. A crevice located at the dimer interface, as well as the results of a site-directed mutagenesis study, led to a model wherein two bleomycin molecules are completely sequestered by one dimer. This provides a novel insight into antibiotic resistance due to drug sequestering, and probably also into drug transport and excretion.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/by/1byl_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1BYL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptoalloteichus_hindustanus Streptoalloteichus hindustanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BYL OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1byl ConSurf].
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Crystal structure and site-directed mutagenesis of a bleomycin resistance protein and their significance for drug sequestering., Dumas P, Bergdoll M, Cagnon C, Masson JM, EMBO J. 1994 Jun 1;13(11):2483-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7516875 7516875]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptoalloteichus hindustanus]]
[[Category: Streptoalloteichus hindustanus]]
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[[Category: Bergdoll, M.]]
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[[Category: Bergdoll M]]
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[[Category: Cagnon, C.]]
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[[Category: Cagnon C]]
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[[Category: Dumas, P.]]
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[[Category: Dumas P]]
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[[Category: Masson, J M.]]
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[[Category: Masson JM]]
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[[Category: antibiotic resistance]]
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[[Category: bleomycin]]
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[[Category: chain swapping]]
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[[Category: drug sequestering]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:17:38 2008''
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Current revision

BLEOMYCIN RESISTANCE PROTEIN FROM STREPTOALLOTEICHUS HINDUSTANUS

PDB ID 1byl

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