4qd2
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Molecular basis for disruption of E-cadherin adhesion by botulinum neurotoxin A complex== | |
| + | <StructureSection load='4qd2' size='340' side='right'caption='[[4qd2]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4qd2]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum_A_str._Hall Clostridium botulinum A str. Hall] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QD2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qd2 OCA], [https://pdbe.org/4qd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qd2 RCSB], [https://www.ebi.ac.uk/pdbsum/4qd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qd2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A5HZZ6_CLOBH A5HZZ6_CLOBH] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | How botulinum neurotoxins (BoNTs) cross the host intestinal epithelial barrier in foodborne botulism is poorly understood. Here, we present the crystal structure of a clostridial hemagglutinin (HA) complex of serotype BoNT/A bound to the cell adhesion protein E-cadherin at 2.4 angstroms. The HA complex recognizes E-cadherin with high specificity involving extensive intermolecular interactions and also binds to carbohydrates on the cell surface. Binding of the HA complex sequesters E-cadherin in the monomeric state, compromising the E-cadherin-mediated intercellular barrier and facilitating paracellular absorption of BoNT/A. We reconstituted the complete 14-subunit BoNT/A complex using recombinantly produced components and demonstrated that abolishing either E-cadherin- or carbohydrate-binding of the HA complex drastically reduces oral toxicity of BoNT/A complex in vivo. Together, these studies establish the molecular mechanism of how HAs contribute to the oral toxicity of BoNT/A. | ||
| - | + | Molecular basis for disruption of E-cadherin adhesion by botulinum neurotoxin A complex.,Lee K, Zhong X, Gu S, Kruel AM, Dorner MB, Perry K, Rummel A, Dong M, Jin R Science. 2014 Jun 20;344(6190):1405-10. doi: 10.1126/science.1253823. PMID:24948737<ref>PMID:24948737</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4qd2" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Cadherin 3D structures|Cadherin 3D structures]] | ||
| + | *[[Hemagglutinin 3D structures|Hemagglutinin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Clostridium botulinum A str. Hall]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Dong M]] | ||
| + | [[Category: Dorner MB]] | ||
| + | [[Category: Gu S]] | ||
| + | [[Category: Jin R]] | ||
| + | [[Category: Kruel A]] | ||
| + | [[Category: Lee K]] | ||
| + | [[Category: Perry K]] | ||
| + | [[Category: Rummel A]] | ||
| + | [[Category: Zhong X]] | ||
Current revision
Molecular basis for disruption of E-cadherin adhesion by botulinum neurotoxin A complex
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Categories: Clostridium botulinum A str. Hall | Large Structures | Mus musculus | Dong M | Dorner MB | Gu S | Jin R | Kruel A | Lee K | Perry K | Rummel A | Zhong X
