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3j3i

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Penicillium chrysogenum virus (PcV) capsid structure

3j3i, resolution 4.10Å

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 ==Penicillium chrysogenum virus (PcV) capsid structure==
-<StructureSection load='3j3i' size='340' side='right' caption='[[3j3i]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
+<SX load='3j3i' size='340' side='right' viewer='molstar' caption='[[3j3i]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3j3i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Penicillium_chrysogenum_virus_(isolate_caston) Penicillium chrysogenum virus (isolate caston)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J3I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J3I FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3j3i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium_chrysogenum_virus_(isolate_Caston) Penicillium chrysogenum virus (isolate Caston)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J3I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J3I FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j3i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j3i RCSB], [http://www.ebi.ac.uk/pdbsum/3j3i PDBsum]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.1&#8491;</td></tr>
-<table>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j3i OCA], [https://pdbe.org/3j3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j3i RCSB], [https://www.ebi.ac.uk/pdbsum/3j3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j3i ProSAT]</span></td></tr>
-<div style="background-color:#fffaf0;">
+</table>
-== Publication Abstract from PubMed ==
+== Function ==
-Viruses evolve so rapidly that sequence-based comparison is not suitable for detecting relatedness among distant viruses. Structure-based comparisons suggest that evolution led to a small number of viral classes or lineages that can be grouped by capsid protein (CP) folds. Here, we report that the CP structure of the fungal dsRNA Penicillium chrysogenum virus (PcV) shows the progenitor fold of the dsRNA virus lineage and suggests a relationship between lineages. Cryo-EM structure at near-atomic resolution showed that the 982-aa PcV CP is formed by a repeated alpha-helical core, indicative of gene duplication despite lack of sequence similarity between the two halves. Superimposition of secondary structure elements identified a single "hotspot" at which variation is introduced by insertion of peptide segments. Structural comparison of PcV and other distantly related dsRNA viruses detected preferential insertion sites at which the complexity of the conserved alpha-helical core, made up of ancestral structural motifs that have acted as a skeleton, might have increased, leading to evolution of the highly varied current structures. Analyses of structural motifs only apparent after systematic structural comparisons indicated that the hallmark fold preserved in the dsRNA virus lineage shares a long (spinal) alpha-helix tangential to the capsid surface with the head-tailed phage and herpesvirus viral lineage.
+[https://www.uniprot.org/uniprot/CAPSD_PCVC CAPSD_PCVC] Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 35-40 nm in diameter.
-Cryo-EM near-atomic structure of a dsRNA fungal virus shows ancient structural motifs preserved in the dsRNA viral lineage.,Luque D, Gomez-Blanco J, Garriga D, Brilot AF, Gonzalez JM, Havens WM, Carrascosa JL, Trus BL, Verdaguer N, Ghabrial SA, Caston JR Proc Natl Acad Sci U S A. 2014 May 27;111(21):7641-6. doi:, 10.1073/pnas.1404330111. Epub 2014 May 12. PMID:24821769<ref>PMID:24821769</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-== References ==
-<references/>
 __TOC__
-</StructureSection>
+</SX>
-[[Category: Brilot, A.]]
+[[Category: Large Structures]]
-[[Category: Carrascosa, J L.]]
+[[Category: Brilot A]]
-[[Category: Caston, J R.]]
+[[Category: Carrascosa JL]]
-[[Category: Garriga, D.]]
+[[Category: Caston JR]]
-[[Category: Ghabrial, S A.]]
+[[Category: Garriga D]]
-[[Category: Gomez-Blanco, J.]]
+[[Category: Ghabrial SA]]
-[[Category: Gonzalez, J M.]]
+[[Category: Gomez-Blanco J]]
-[[Category: Grigorieff, N.]]
+[[Category: Gonzalez JM]]
-[[Category: Havens, W H.]]
+[[Category: Grigorieff N]]
-[[Category: Luque, D.]]
+[[Category: Havens WH]]
-[[Category: Trus, B L.]]
+[[Category: Luque D]]
-[[Category: Verdaguer, N.]]
+[[Category: Trus BL]]
-[[Category: Double-stranded rna]]
+[[Category: Verdaguer N]]
-[[Category: Duplicated helical fold]]
-[[Category: Fungal virus]]
-[[Category: T=1 virus]]
-[[Category: Viral structure]]
-[[Category: Virus]]

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Penicillium chrysogenum virus (PcV) capsid structure

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