Exoenzyme
From Proteopedia
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(New page: <StructureSection load='2c8c' size='340' side='right' caption='Structure of exoenzyme C3 catalytic domain complex with ADP and NAD (PDB code 2c8c).' scene=''> == Function == '''Exoe...) |
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| + | <StructureSection load='2c8c' size='400' side='right' caption='Structure of exoenzyme C3 catalytic domain complex with ADP and NAD (PDB code [[2c8c]]).' scene='59/593306/Cv/8'> | ||
| - | + | '''Exoenzymes''' (Exo) are secreted by cells and break large macromolecules outside the cells. The breakdown components can pass through the cell wall. | |
| + | *'''Exo C3''' is a ''Clostridium butulinum'' toxin which binds [[NAD]], splits it into ADP-ribose and nicotinamide and transfers the ADP-ribose to a protein substrate. | ||
| + | * '''Exo S''' and '''Exo T''' are secreted by ''Pseudomonas aeruginosa'' and has GTPase-activating activity as well as ADP-ribosyltransferase activity<ref>PMID:11114250</ref> . | ||
| - | = | + | <scene name='59/593306/Cv/6'>NAD binding site</scene>. |
| - | '' | + | <scene name='59/593306/Cv/7'>ADP binding site</scene> in exoenzyme C3 catalytic domain (PDB code [[2c8c]]). <ref>PMID:18369192</ref> |
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==3D structures of exoenzyme== | ==3D structures of exoenzyme== | ||
| + | [[Exoenzyme 3D structures]] | ||
| - | + | </StructureSection> | |
| - | '''Exoenzyme C3''' | ||
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| - | [[1g24]] – CbExo residues 8-218 – ''Clostridium butulinum''<br /> | ||
| - | [[1uzi]], [[1r45]], [[1r4b]], [[2c89]] – CbExo catalytic domain <br /> | ||
| - | [[1gze]], [[2c8b]], [[2c8d]], [[2c8e]], [[2c8g]] – CbExo catalytic domain (mutant)<br /> | ||
| - | [[3bw8]] - Exo catalytic domain - ''Clostridium limosum''<br /> | ||
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| - | ''Exoenzyme C3 complexes'' | ||
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| - | [[1gzf]] – CbExo catalytic domain + pyridinium derivative + ADP + NAD<br /> | ||
| - | [[2bov]] – CbExo + GDP + Ral-A GTPase<br /> | ||
| - | [[2a78]] – CbExo catalytic domain + GDP + Ral-A GTPase<br /> | ||
| - | [[2a9k]] – CbExo catalytic domain+ GDP + Ral-A GTPase + NAD<br /> | ||
| - | [[2c8a]] – CbExo catalytic domain + nicotinamide<br /> | ||
| - | [[2c8c]] – CbExo catalytic domain (mutant) + ADP + NAD<br /> | ||
| - | [[2c8f]], [[2c8h]] – CbExo catalytic domain (mutant) + NAD<br /> | ||
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| - | '''Exoenzyme S''' | ||
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| - | [[1r4t]] – PaExo GAP domain – ''Pseudomonas aeruginosa'' - NMR<br /> | ||
| - | [[1he1]] – PaExo GAP domain + Ras-like protein + GDP <br /> | ||
| - | [[1he9]] – PaExo GAP domain + Ras-like protein <br /> | ||
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| - | '''Exoenzyme T''' | ||
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| - | [[4jmf]] – PaExo residues 28-77 + chaperone<br /> | ||
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| - | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
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References
- ↑ Han S, Arvai AS, Clancy SB, Tainer JA. Crystal structure and novel recognition motif of rho ADP-ribosylating C3 exoenzyme from Clostridium botulinum: structural insights for recognition specificity and catalysis. J Mol Biol. 2001 Jan 5;305(1):95-107. PMID:11114250 doi:10.1006/jmbi.2000.4292
- ↑ Menetrey J, Flatau G, Boquet P, Menez A, Stura EA. Structural basis for the NAD-hydrolysis mechanism and the ARTT-loop plasticity of C3 exoenzymes. Protein Sci. 2008 May;17(5):878-86. Epub 2008 Mar 27. PMID:18369192 doi:10.1110/ps.073398508
