4tv6
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4tv6 is ON HOLD Authors: Kobylarz, M.J., Grigg, J.C., Murphy, M.E.P. Description: Crystal Structure of Citrate Synthase Variant SbnG E151Q) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of Citrate Synthase Variant SbnG E151Q== | |
| + | <StructureSection load='4tv6' size='340' side='right'caption='[[4tv6]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4tv6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_str._Newman Staphylococcus aureus subsp. aureus str. Newman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TV6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OAA:OXALOACETATE+ION'>OAA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tv6 OCA], [https://pdbe.org/4tv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tv6 RCSB], [https://www.ebi.ac.uk/pdbsum/4tv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tv6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0H3K9Z0_STAAE A0A0H3K9Z0_STAAE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In response to iron deprivation, Staphylococcus aureus produces staphyloferrin B, a citrate-containing siderophore that delivers iron back to the cell. This bacterium also possesses a second citrate synthase, SbnG, that is necessary for supplying citrate to the staphyloferrin B biosynthetic pathway. We present the structure of SbnG bound to the inhibitor calcium and an active site variant in complex with oxaloacetate. The overall fold of SbnG is structurally distinct from TCA cycle citrate synthases, yet similar to metal-dependent class II aldolases. Phylogenetic analyses revealed that SbnG forms a separate clade with homologs from other siderophore biosynthetic gene clusters and is representative of a metal-independent subgroup in the phosphoenolpyruvate/pyruvate domain superfamily. A structural superposition of the SbnG active site to TCA cycle citrate synthases and site-directed mutagenesis suggests a case for convergent evolution towards a conserved catalytic mechanism for citrate production. | ||
| - | + | SbnG, a Citrate Synthase in Staphylococcus aureus: a New Fold on an Old Enzyme.,Kobylarz MJ, Grigg JC, Sheldon JR, Heinrichs DE, Murphy ME J Biol Chem. 2014 Oct 21. pii: jbc.M114.603175. PMID:25336653<ref>PMID:25336653</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4tv6" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Staphylococcus aureus subsp. aureus str. Newman]] | ||
| + | [[Category: Grigg JC]] | ||
| + | [[Category: Kobylarz MJ]] | ||
| + | [[Category: Murphy MEP]] | ||
Current revision
Crystal Structure of Citrate Synthase Variant SbnG E151Q
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