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4qed

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==ElxO Y152F with NADPH Bound==
==ElxO Y152F with NADPH Bound==
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<StructureSection load='4qed' size='340' side='right' caption='[[4qed]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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<StructureSection load='4qed' size='340' side='right'caption='[[4qed]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4qed]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QED OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QED FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4qed]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QED OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QED FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qec|4qec]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qed FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qed OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qed RCSB], [http://www.ebi.ac.uk/pdbsum/4qed PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qed FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qed OCA], [https://pdbe.org/4qed PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qed RCSB], [https://www.ebi.ac.uk/pdbsum/4qed PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qed ProSAT]</span></td></tr>
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<table>
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</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/I6ZQW6_STAEP I6ZQW6_STAEP]
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The final step in lanthipeptide biosynthesis involves the proteolytic removal of an N-terminal leader peptide. In the class I lanthipeptide epilancin 15X, this step is performed by the subtilisin-like serine peptidase ElxP. Bioinformatic, kinetic, and mass spectrometric analysis revealed that ElxP recognizes the stretch of amino acids DLNPQS located near the proteolytic cleavage site of its substrate, ElxA. When the ElxP recognition motif was inserted into the noncognate lanthipeptide precursor NisA, ElxP was able to proteolytically remove the leader peptide from NisA. Proteolytic removal of the leader peptide by ElxP during the biosynthesis of epilancin 15X exposes an N-terminal dehydroalanine on the core peptide of ElxA that hydrolyzes to a pyruvyl group. The short-chain dehydrogenase ElxO reduces the pyruvyl group to a lactyl moiety in the final step of epilancin 15X maturation. Using synthetic peptides, we also investigated the substrate specificity of ElxO and determined the 1.85 A resolution X-ray crystal structure of the enzyme.
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Substrate Specificity of the Lanthipeptide Peptidase ElxP and the Oxidoreductase ElxO.,Ortega MA, Velasquez JE, Garg N, Zhang Q, Joyce RE, Nair SK, van der Donk WA ACS Chem Biol. 2014 Jun 6. PMID:24866416<ref>PMID:24866416</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Garg, N.]]
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[[Category: Large Structures]]
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[[Category: Nair, S K.]]
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[[Category: Staphylococcus epidermidis]]
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[[Category: Oxidoreductase]]
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[[Category: Garg N]]
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[[Category: Rossmann fold]]
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[[Category: Nair SK]]

Current revision

ElxO Y152F with NADPH Bound

PDB ID 4qed

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