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3wa1
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| - | ==Crystal structure of | + | |
| - | <StructureSection load='3wa1' size='340' side='right' caption='[[3wa1]], [[Resolution|resolution]] 1.75Å' scene=''> | + | ==Crystal structure of BinB: A receptor binding component of the binary toxin from Lysinibacillus sphaericus== |
| + | <StructureSection load='3wa1' size='340' side='right'caption='[[3wa1]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3wa1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA1 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[3wa1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_14577 Atcc 14577]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WA1 FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">binB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1421 ATCC 14577])</td></tr> |
| - | <table> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wa1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa1 OCA], [https://pdbe.org/3wa1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wa1 RCSB], [https://www.ebi.ac.uk/pdbsum/3wa1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wa1 ProSAT]</span></td></tr> |
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The binary toxin (Bin), produced by Lysinibacillus sphaericus, is composed of BinA (42 kDa) and BinB (51 kDa) proteins, which are both required for full toxicity against Culex and Anopheles mosquito larvae. Specificity of Bin toxin is determined by the binding of BinB component to a receptor present on the midgut epithelial membranes, while BinA is proposed to be a toxic component. Here, we determined the first crystal structure of the active form of BinB at a resolution of 1.75 A. BinB possesses two distinct structural domains in its N- and C-termini. The globular N-terminal domain has a beta-trefoil scaffold which is a highly conserved architecture of some sugar binding proteins or lectins, suggesting a role of this domain in receptor-binding. The BinB beta-rich C-terminal domain shares similar three-dimensional folding with aerolysin type beta-pore forming toxins, despite a low sequence identity. The BinB structure, therefore, is a new member of the aerolysin-like toxin family, with probably similarities in the cytolytic mechanism that takes place via pore formation. Proteins 2014; 82:2703-2712. (c) 2014 Wiley Periodicals, Inc. | ||
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| + | Crystal structure of BinB: A receptor binding component of the binary toxin from Lysinibacillus sphaericus.,Srisucharitpanit K, Yao M, Promdonkoy B, Chimnaronk S, Tanaka I, Boonserm P Proteins. 2014 Oct;82(10):2703-12. doi: 10.1002/prot.24636. Epub 2014 Jul 5. PMID:24975613<ref>PMID:24975613</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3wa1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Boonserm, P | + | [[Category: Atcc 14577]] |
| - | [[Category: Chimnaronk, S | + | [[Category: Large Structures]] |
| - | [[Category: Promdonkoy, B | + | [[Category: Boonserm, P]] |
| - | [[Category: Srisucharitpanit, K | + | [[Category: Chimnaronk, S]] |
| - | [[Category: Tanaka, I | + | [[Category: Promdonkoy, B]] |
| - | [[Category: Yao, M | + | [[Category: Srisucharitpanit, K]] |
| + | [[Category: Tanaka, I]] | ||
| + | [[Category: Yao, M]] | ||
[[Category: A-b toxin]] | [[Category: A-b toxin]] | ||
[[Category: Binary toxin]] | [[Category: Binary toxin]] | ||
[[Category: Toxin]] | [[Category: Toxin]] | ||
Current revision
Crystal structure of BinB: A receptor binding component of the binary toxin from Lysinibacillus sphaericus
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