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1cfe

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[[Image:1cfe.jpg|left|200px]]
 
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{{Structure
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==P14A, NMR, 20 STRUCTURES==
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|PDB= 1cfe |SIZE=350|CAPTION= <scene name='initialview01'>1cfe</scene>
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<StructureSection load='1cfe' size='340' side='right'caption='[[1cfe]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1cfe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_lycopersicum Solanum lycopersicum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CFE FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfe OCA], [https://pdbe.org/1cfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cfe RCSB], [https://www.ebi.ac.uk/pdbsum/1cfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfe ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''P14A, NMR, 20 STRUCTURES'''
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[https://www.uniprot.org/uniprot/PR06_SOLLC PR06_SOLLC] Probably involved in the defense reaction of plants against pathogens. Has antifungal activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The nuclear magnetic resonance (NMR) structure of the 15 kDa pathogenesis-related protein P14a, which displays antifungicidal activity and is induced in tomato leaves as a response to pathogen infection, was determined using 15N/13C doubly labeled and unlabeled protein samples. In all, 2030 conformational constraints were collected as input for the distance geometry program DIANA. After energy-minimization with the program OPAL the 20 best conformers had an average root-mean-square deviation value relative to the mean coordinates of 0.88 A for the backbone atoms N, C(alpha) and C', and 1.30 A for all heavy atoms. P14a contains four alpha-helices (I to IV) comprising residues 4 to 17, 27 to 40, 64 to 72 and 93 to 98, a short 3(10)-helix of residues 73 to 75 directly following helix III, and a mixed, four-stranded beta-sheet with topology +3x, -2x, +1, containing the residues 24-25, 53 to 58, 104 to 111 and 117 to 124. These regular secondary structure elements form a novel, complex alpha + beta topology in which the alpha-helices I, III and IV and the 3(10)-helix are located above the plane defined by the beta-sheet, and the alpha-helix II lies below this plane. The alpha-helices and beta-strands are thus arranged in three stacked layers, which are stabilized by two distinct hydrophobic cores associated with the two layer interfaces, giving rise to an "alpha-beta-alpha sandwich". The three-dimensional structure of P14a provides initial leads for identification of the so far unknown active sites and the mode of action of the protein, which is of direct interest for the generation of transgenic plants with improved host defense properties.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/1cfe_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1CFE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_lycopersicum Solanum lycopersicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFE OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cfe ConSurf].
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NMR solution structure of the pathogenesis-related protein P14a., Fernandez C, Szyperski T, Bruyere T, Ramage P, Mosinger E, Wuthrich K, J Mol Biol. 1997 Feb 28;266(3):576-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9067611 9067611]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Solanum lycopersicum]]
[[Category: Solanum lycopersicum]]
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[[Category: Bruyere, T.]]
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[[Category: Bruyere T]]
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[[Category: Fernandez, C.]]
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[[Category: Fernandez C]]
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[[Category: Mosinger, E.]]
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[[Category: Mosinger E]]
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[[Category: Ramage, P.]]
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[[Category: Ramage P]]
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[[Category: Szyperski, T.]]
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[[Category: Szyperski T]]
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[[Category: Wuthrich, K.]]
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[[Category: Wuthrich K]]
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[[Category: pathogenesis-related protein]]
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[[Category: plant defense]]
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[[Category: pr-1 protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:23:49 2008''
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Current revision

P14A, NMR, 20 STRUCTURES

PDB ID 1cfe

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