4dn3

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of anti-mcp-1 antibody cnto888

Structural highlights

4dn3 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Human CC chemokine ligand 2 (CCL2), also known as monocyte chemoattractant protein-1 (MCP-1), is a member of the beta chemokine family whose actions are mediated through the G-protein-coupled receptor CCR2. Binding of CCL2 to its receptor CCR2 triggers calcium mobilization and chemotaxis. CCL2 is implicated in the pathogenesis of certain inflammatory diseases and cancer. CNTO 888, a neutralizing human anti-CCL2 antibody, was derived by antibody phage display. The antibody binds human CCL2 with high affinity (K(D)=22 pM) and inhibits CCL2 binding to its receptor. The crystal structure of the CNTO 888 Fab alone and in complex with the monomeric form of CCL2 (P8A variant) was determined at 2.6 A and 2.8 A resolution, respectively. CNTO 888 recognizes a conformational epitope encompassing residues 18-24 and 45-51 that overlaps the mapped receptor binding site. The epitope of CNTO 888 does not overlap with the dimerization site of CCL2, and thus its inhibitory activity is not expected to result from interference with the oligomeric state of CCL2. Comparison of the X-ray-determined epitopes of CNTO 888 and another CCL2-neutralizing antibody, 11K2, provides insight into the molecular basis of antibody selectivity and functional inhibition.

Structural basis for high selectivity of anti-CCL2 neutralizing antibody CNTO 888.,Obmolova G, Teplyakov A, Malia TJ, Grygiel TL, Sweet R, Snyder LA, Gilliland GL Mol Immunol. 2012 Jun;51(2):227-33. Epub 2012 Apr 7. PMID:22487721^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Obmolova G, Teplyakov A, Malia TJ, Grygiel TL, Sweet R, Snyder LA, Gilliland GL. Structural basis for high selectivity of anti-CCL2 neutralizing antibody CNTO 888. Mol Immunol. 2012 Jun;51(2):227-33. Epub 2012 Apr 7. PMID:22487721 doi:http://dx.doi.org/10.1016/j.molimm.2012.03.022

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4dn3"

@@ Line 1: / Line 1: @@
 ==Crystal structure of anti-mcp-1 antibody cnto888==
-<StructureSection load='4dn3' size='340' side='right' caption='[[4dn3]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+<StructureSection load='4dn3' size='340' side='right'caption='[[4dn3]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4dn3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DN3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DN3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4dn3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DN3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DN3 FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4dn4|4dn4]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dn3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dn3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dn3 RCSB], [http://www.ebi.ac.uk/pdbsum/4dn3 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dn3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dn3 OCA], [https://pdbe.org/4dn3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dn3 RCSB], [https://www.ebi.ac.uk/pdbsum/4dn3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dn3 ProSAT]</span></td></tr>
-<table>
+</table>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 14: / Line 15: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4dn3" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 19: / Line 21: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Gilliland, G.]]
+[[Category: Large Structures]]
-[[Category: Grygiel, T.]]
+[[Category: Gilliland G]]
-[[Category: L., Snyder.]]
+[[Category: Grygiel T]]
-[[Category: Malia, T.]]
+[[Category: Malia T]]
-[[Category: Obmolova, G.]]
+[[Category: Obmolova G]]
-[[Category: Sweet, R.]]
+[[Category: Snyder L]]
-[[Category: Teplyakov, A.]]
+[[Category: Sweet R]]
-[[Category: Antibody]]
+[[Category: Teplyakov A]]
-[[Category: Immune system]]

4dn3

From Proteopedia

Current revision

Crystal structure of anti-mcp-1 antibody cnto888

Structural highlights

Publication Abstract from PubMed

References

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