4qvg
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4qvg is ON HOLD Authors: Liu, J.S., Chen, S. C., Huang, C. H., Yang, C. S., Chen, Y. Description: Crystal structure of S-adenosylmethionine-depende...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of S-adenosylmethionine-dependent methyltransferase SibL in its apo form== | |
| + | <StructureSection load='4qvg' size='340' side='right'caption='[[4qvg]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4qvg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptosporangium_sibiricum Streptosporangium sibiricum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QVG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QVG FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qvg OCA], [https://pdbe.org/4qvg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qvg RCSB], [https://www.ebi.ac.uk/pdbsum/4qvg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qvg ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/C0LTM6_STRSJ C0LTM6_STRSJ] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Streptosporangium sibiricum SibL catalyzes the methyl transfer from S-adenosylmethionine (SAM) to 3-hydroxykynurenine (3-HK) to produce S-adenosylhomocysteine (SAH) and 3-hydroxy-4-methyl-kynurenine for sibiromycin biosynthesis. Here, we present the crystal structures of apo-form Ss-SibL, Ss-SibL/SAH binary complex and Ss-SibL/SAH/3-HK ternary complex. Ss-SibL is a homodimer. Each subunit comprises a helical N-terminal domain and a Rossmann-fold C-terminal domain. SAM (or SAH) binding alone results in domain movements, suggesting a two-step catalytic cycle. Analyses of the enzyme-ligand interactions and further mutant studies support a mechanism in which Tyr134 serves as the principal base in the transferase reaction of methyl group from SAM to 3-HK. | ||
| - | + | Structure and mechanism of an antibiotics-synthesizing 3-hydroxykynurenine C-methyltransferase.,Chen SC, Huang CH, Lai SJ, Liu JS, Fu PK, Tseng ST, Yang CS, Lai MC, Ko TP, Chen Y Sci Rep. 2015 May 11;5:10100. doi: 10.1038/srep10100. PMID:25960001<ref>PMID:25960001</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4qvg" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptosporangium sibiricum]] | ||
| + | [[Category: Chen SC]] | ||
| + | [[Category: Chen Y]] | ||
| + | [[Category: Huang CH]] | ||
| + | [[Category: Liu JS]] | ||
| + | [[Category: Yang CS]] | ||
Current revision
Crystal structure of S-adenosylmethionine-dependent methyltransferase SibL in its apo form
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