This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3syl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:12, 14 March 2024) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal structure of the AAA+ protein CbbX, native structure==
==Crystal structure of the AAA+ protein CbbX, native structure==
-
<StructureSection load='3syl' size='340' side='right' caption='[[3syl]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+
<StructureSection load='3syl' size='340' side='right'caption='[[3syl]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3syl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhodococcus_capsulatus"_molisch_1907 "rhodococcus capsulatus" molisch 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SYL FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3syl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SYL FirstGlance]. <br>
-
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
-
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3syk|3syk]], [[3zuh|3zuh]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cbbX, RSKD131_2679 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 "Rhodococcus capsulatus" Molisch 1907])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3syl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syl OCA], [https://pdbe.org/3syl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3syl RCSB], [https://www.ebi.ac.uk/pdbsum/3syl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3syl ProSAT]</span></td></tr>
-
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3syl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3syl RCSB], [http://www.ebi.ac.uk/pdbsum/3syl PDBsum]</span></td></tr>
+
</table>
-
<table>
+
== Function ==
-
<div style="background-color:#fffaf0;">
+
[https://www.uniprot.org/uniprot/CBBX_CERSP CBBX_CERSP] ATPase involved in the activation of red-type RuBisCo (ribulose-1,5-bisphosphate carboxylase/oxygenase), which tends to form inactive complexes with its substrate ribulose 1,5-bisphosphate (RuBP) (PubMed:27872295, PubMed:22048315). Catalyzes the release of RuBP from inhibited RuBisCo in an ATP-dependent manner (PubMed:27872295, PubMed:22048315). Activation of RuBisCO involves the ATP-dependent carboxylation of the epsilon-amino group of lysine leading to a carbamate structure (PubMed:27872295, PubMed:22048315).<ref>PMID:22048315</ref> <ref>PMID:27872295</ref>
-
== Publication Abstract from PubMed ==
+
-
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyses the fixation of atmospheric CO(2) in photosynthesis, but tends to form inactive complexes with its substrate ribulose 1,5-bisphosphate (RuBP). In plants, Rubisco is reactivated by the AAA(+) (ATPases associated with various cellular activities) protein Rubisco activase (Rca), but no such protein is known for the Rubisco of red algae. Here we identify the protein CbbX as an activase of red-type Rubisco. The 3.0-A crystal structure of unassembled CbbX from Rhodobacter sphaeroides revealed an AAA(+) protein architecture. Electron microscopy and biochemical analysis showed that ATP and RuBP must bind to convert CbbX into functionally active, hexameric rings. The CbbX ATPase is strongly stimulated by RuBP and Rubisco. Mutational analysis suggests that CbbX functions by transiently pulling the carboxy-terminal peptide of the Rubisco large subunit into the hexamer pore, resulting in the release of the inhibitory RuBP. Understanding Rubisco activation may facilitate efforts to improve CO(2) uptake and biomass production by photosynthetic organisms.
+
-
 
+
-
Structure and function of the AAA+ protein CbbX, a red-type Rubisco activase.,Mueller-Cajar O, Stotz M, Wendler P, Hartl FU, Bracher A, Hayer-Hartl M Nature. 2011 Nov 2;479(7372):194-9. doi: 10.1038/nature10568. PMID:22048315<ref>PMID:22048315</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Rhodococcus capsulatus molisch 1907]]
+
[[Category: Cereibacter sphaeroides]]
-
[[Category: Bracher, A.]]
+
[[Category: Large Structures]]
-
[[Category: Hartl, F U.]]
+
[[Category: Bracher A]]
-
[[Category: Hayer-Hartl, M.]]
+
[[Category: Hartl FU]]
-
[[Category: Mueller-Cajar, O.]]
+
[[Category: Hayer-Hartl M]]
-
[[Category: Stotz, M.]]
+
[[Category: Mueller-Cajar O]]
-
[[Category: Wendler, P.]]
+
[[Category: Stotz M]]
-
[[Category: Aaa+ protein]]
+
[[Category: Wendler P]]
-
[[Category: Calvin cycle]]
+
-
[[Category: Chaperone]]
+
-
[[Category: Photosynthesis]]
+
-
[[Category: Rubisco activase]]
+

Current revision

Crystal structure of the AAA+ protein CbbX, native structure

PDB ID 3syl

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3syl"
Personal tools