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1d7a

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[[Image:1d7a.jpg|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF E. COLI PURE-MONONUCLEOTIDE COMPLEX.==
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|PDB= 1d7a |SIZE=350|CAPTION= <scene name='initialview01'>1d7a</scene>, resolution 2.5&Aring;
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<StructureSection load='1d7a' size='340' side='right'caption='[[1d7a]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=AIR:5-AMINOIMIDAZOLE RIBONUCLEOTIDE'>AIR</scene>
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<table><tr><td colspan='2'>[[1d7a]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D7A FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Phosphoribosylaminoimidazole_carboxylase Phosphoribosylaminoimidazole carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.21 4.1.1.21]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AIR:5-AMINOIMIDAZOLE+RIBONUCLEOTIDE'>AIR</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d7a OCA], [https://pdbe.org/1d7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d7a RCSB], [https://www.ebi.ac.uk/pdbsum/1d7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d7a ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PURE_ECOLI PURE_ECOLI] Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d7/1d7a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d7a ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF E. COLI PURE-MONONUCLEOTIDE COMPLEX.'''
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==See Also==
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*[[Phosphoribosylaminoimidazole carboxylase|Phosphoribosylaminoimidazole carboxylase]]
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*[[Phosphoribosylaminoimidazole carboxylase 3D structures|Phosphoribosylaminoimidazole carboxylase 3D structures]]
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==Overview==
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__TOC__
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BACKGROUND: Conversion of 5-aminoimidazole ribonucleotide (AIR) to 4-carboxyaminoimidazole ribonucleotide (CAIR) in Escherichia coli requires two proteins - PurK and PurE. PurE has recently been shown to be a mutase that catalyzes the unusual rearrangement of N(5)-carboxyaminoimidazole ribonucleotide (N(5)-CAIR), the PurK reaction product, to CAIR. PurEs from higher eukaryotes are homologous to E. coli PurE, but use AIR and CO(2) as substrates to produce CAIR directly. RESULTS: The 1.50 A crystal structure of PurE reveals an octameric structure with 422 symmetry. A central three-layer (alphabetaalpha) sandwich domain and a kinked C-terminal helix form the folded structure of the monomeric unit. The structure reveals a cleft at the interface of two subunits and near the C-terminal helix of a third subunit. Co-crystallization experiments with CAIR confirm this to be the mononucleotide-binding site. The nucleotide is bound predominantly to one subunit, with conserved residues from a second subunit making up one wall of the cleft. CONCLUSIONS: The crystal structure of PurE reveals a unique quaternary structure that confirms the octameric nature of the enzyme. An analysis of the native crystal structure, in conjunction with sequence alignments and studies of co-crystals of PurE with CAIR, reveals the location of the active site. The environment of the active site and the analysis of conserved residues between the two classes of PurEs suggests a model for the differences in their substrate specificities and the relationship between their mechanisms.
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</StructureSection>
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==About this Structure==
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1D7A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D7A OCA].
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==Reference==
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Crystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway., Mathews II, Kappock TJ, Stubbe J, Ealick SE, Structure. 1999 Nov 15;7(11):1395-406. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10574791 10574791]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Phosphoribosylaminoimidazole carboxylase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Ealick SE]]
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[[Category: Ealick, S E.]]
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[[Category: Kappock TJ]]
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[[Category: Kappock, T J.]]
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[[Category: Mathews II]]
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[[Category: Mathews, I I.]]
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[[Category: Stubbe J]]
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[[Category: Stubbe, J.]]
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[[Category: AIR]]
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[[Category: aminoimidazole ribonucleotide (air)]]
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[[Category: three-layer (alpha-beta-alpha) sandwich n5-cair mutase (pure)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:33:51 2008''
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Current revision

CRYSTAL STRUCTURE OF E. COLI PURE-MONONUCLEOTIDE COMPLEX.

PDB ID 1d7a

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