Sandbox bcce16
From Proteopedia
(Difference between revisions)
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<scene name='59/596450/Bmori_pbp_ser56/1'>serine 56,</scene> forms a hydrogen bond with the hydroxyl functional group of the pheromone; this is mutated to alanine in lepidopteran species which use acetyl esters as pheromones. Some species with multiple pheromone components possess multiple PBP isoforms, suggesting a role in odorant discrimination as well. | <scene name='59/596450/Bmori_pbp_ser56/1'>serine 56,</scene> forms a hydrogen bond with the hydroxyl functional group of the pheromone; this is mutated to alanine in lepidopteran species which use acetyl esters as pheromones. Some species with multiple pheromone components possess multiple PBP isoforms, suggesting a role in odorant discrimination as well. | ||
| - | Once the PBP reaches the odorant receptor at the neuronal membrane, it ejects the bound pheromone molecule via a pH-dependent conformational change. In the binding-competent high-pH form, the C-terminal residues 131-142 form a disordered surface loop. But in the non-binding low-pH form, these residues form an <scene name='59/596450/Bmori_pbp_closed/ | + | Once the PBP reaches the odorant receptor at the neuronal membrane, it ejects the bound pheromone molecule via a pH-dependent conformational change. In the binding-competent high-pH form, the C-terminal residues 131-142 form a <scene name='59/596450/Bmori_pbp_open/1'>disordered surface loop</scene>. But in the non-binding low-pH form, these residues form an <scene name='59/596450/Bmori_pbp_closed/4'>alpha helix</scene> that fills the ligand binding site. |
Current revision
Bombyx mori pheromone binding protein (PBP)
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References
- ↑ Sandler BH, Nikonova L, Leal WS, Clardy J. Sexual attraction in the silkworm moth: structure of the pheromone-binding-protein-bombykol complex. Chem Biol. 2000 Feb;7(2):143-51. PMID:10662696
- ↑ Horst R, Damberger F, Luginbuhl P, Guntert P, Peng G, Nikonova L, Leal WS, Wuthrich K. NMR structure reveals intramolecular regulation mechanism for pheromone binding and release. Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14374-9. Epub 2001 Nov 27. PMID:11724947 doi:10.1073/pnas.251532998
