4u6v
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4u6v is ON HOLD Authors: Oganesyan, V.Y., Peng, L., Damschroder, M.M., Cheng, L., Sadowska, A., Tkaczyk, C., Sellman, B., Wu, H., Dall'Acqua, W.F. ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Mechanisms of Neutralization of a Human Anti-Alpha Toxin Antibody== | |
| + | <StructureSection load='4u6v' size='340' side='right'caption='[[4u6v]], [[Resolution|resolution]] 2.56Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4u6v]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_TCH60 Staphylococcus aureus subsp. aureus TCH60]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U6V FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.56Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u6v OCA], [https://pdbe.org/4u6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u6v RCSB], [https://www.ebi.ac.uk/pdbsum/4u6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u6v ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/HLA_STAA8 HLA_STAA8] Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | MEDI4893 is a neutralizing human monoclonal antibody that targets alpha toxin (AT), and is currently undergoing evaluation in the field of Staphylococcus aureus-mediated diseases. We have solved the crystal structure of MEDI4893 Fab bound to monomeric AT at a resolution of 2.56 A, and further characterized its epitope using various engineered AT variants. We have found that MEDI4893 recognizes a novel epitope in AT so-called rim domain, and exerts its neutralizing effect through a dual mechanism. More particularly, MEDI4893 not only sterically blocks binding of AT to its cell receptor, but also prevents it from adopting a lytic heptameric trans-membrane conformation. | ||
| - | + | Mechanisms of Neutralization of a Human Anti-Alpha Toxin Antibody.,Oganesyan V, Peng L, Damschroder MM, Cheng L, Sadowska A, Tkaczyk C, Sellman BR, Wu H, Dall'Acqua WF J Biol Chem. 2014 Sep 10. pii: jbc.M114.601328. PMID:25210036<ref>PMID:25210036</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4u6v" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Hemolysin 3D structures|Hemolysin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Staphylococcus aureus subsp. aureus TCH60]] | ||
| + | [[Category: Cheng L]] | ||
| + | [[Category: Dall'Acqua WF]] | ||
| + | [[Category: Damschroder MM]] | ||
| + | [[Category: Oganesyan VY]] | ||
| + | [[Category: Peng L]] | ||
| + | [[Category: Sadowska A]] | ||
| + | [[Category: Sellman B]] | ||
| + | [[Category: Tkaczyk C]] | ||
| + | [[Category: Wu H]] | ||
Current revision
Mechanisms of Neutralization of a Human Anti-Alpha Toxin Antibody
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