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1dbw

From Proteopedia

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[[Image:1dbw.jpg|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF FIXJ-N==
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|PDB= 1dbw |SIZE=350|CAPTION= <scene name='initialview01'>1dbw</scene>, resolution 1.60&Aring;
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<StructureSection load='1dbw' size='340' side='right'caption='[[1dbw]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=15P:POLYETHYLENE GLYCOL (N=34)'>15P</scene>
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<table><tr><td colspan='2'>[[1dbw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DBW FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [https://pdbe.org/1dbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [https://www.ebi.ac.uk/pdbsum/1dbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbw ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF FIXJ-N'''
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== Function ==
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[https://www.uniprot.org/uniprot/FIXJ_RHIME FIXJ_RHIME] FixJ, when activated by FixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for FixN activation.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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BACKGROUND: Two-component signal transduction pathways are sophisticated phosphorelay cascades widespread in prokaryotes and also found in fungi, molds and plants. FixL/FixJ is a prototypical system responsible for the regulation of nitrogen fixation in the symbiotic bacterium Sinorhizobium meliloti. In microaerobic conditions the membrane-bound kinase FixL uses ATP to transphosphorylate a histidine residue, and the response regulator FixJ transfers the phosphoryl group from the phosphohistidine to one of its own aspartate residues in a Mg(2+)-dependent mechanism. RESULTS: Seven X-ray structures of the unphosphorylated N-terminal receiver domain of FixJ (FixJN) have been solved from two crystal forms soaked in different conditions. Three conformations of the protein were found. In the first case, the protein fold impairs metal binding in the active site and the structure reveals a receiver domain that is self-inhibited for catalysis. In the second conformation, the canonical geometry of the active site is attained, and subsequent metal binding to the protein induces minimal conformational changes. The third conformation illustrates a non-catalytic form of the protein where unwinding of the N terminus of helix alpha 1 has occurred. Interconversion of the canonical and self-inhibited conformations requires a large conformational change of the beta 3-alpha 3 loop region. CONCLUSIONS: These unphosphorylated structures of FixJN stress the importance of flexible peptide segments that delineate the active site. Their movements may act as molecular switches that define the functional status of the protein. Such observations are in line with structural and biochemical results obtained on other response regulator proteins and may illustrate general features that account for the specificity of protein-protein interactions.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/db/1dbw_consurf.spt"</scriptWhenChecked>
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1DBW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structural transitions in the FixJ receiver domain., Gouet P, Fabry B, Guillet V, Birck C, Mourey L, Kahn D, Samama JP, Structure. 1999 Dec 15;7(12):1517-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10647182 10647182]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dbw ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Sinorhizobium meliloti]]
[[Category: Sinorhizobium meliloti]]
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[[Category: Birck, C.]]
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[[Category: Birck C]]
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[[Category: Fabry, B.]]
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[[Category: Fabry B]]
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[[Category: Gouet, P.]]
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[[Category: Gouet P]]
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[[Category: Guillet, V.]]
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[[Category: Guillet V]]
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[[Category: Kahn, D.]]
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[[Category: Kahn D]]
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[[Category: Mourey, L.]]
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[[Category: Mourey L]]
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[[Category: Samama, J P.]]
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[[Category: Samama JP]]
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[[Category: 15P]]
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[[Category: doubly wound five-stranded beta/alpha fold]]
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[[Category: nitrogen fixation regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:36:00 2008''
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Current revision

CRYSTAL STRUCTURE OF FIXJ-N

PDB ID 1dbw

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