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1ddp

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[[Image:1ddp.gif|left|200px]]
 
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{{Structure
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==Solution structure of a CISPLATIN-INDUCED [CATAGCTATG]2 Interstrand cross-link==
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|PDB= 1ddp |SIZE=350|CAPTION= <scene name='initialview01'>1ddp</scene>
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<StructureSection load='1ddp' size='340' side='right'caption='[[1ddp]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CPT:CIS-PLATINUM-(NH3)2'>CPT</scene>
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<table><tr><td colspan='2'>[[1ddp]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DDP FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CPT:CISPLATIN'>CPT</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ddp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ddp OCA], [https://pdbe.org/1ddp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ddp RCSB], [https://www.ebi.ac.uk/pdbsum/1ddp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ddp ProSAT]</span></td></tr>
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</table>
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'''SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK'''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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==Overview==
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[[Category: Drobny GP]]
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The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin or cis-DDP) reacts with DNA, cross-linking two purine residues through the N7 atoms, which reside in the major groove in B-form DNA. The solution structure of the short duplex [d(CAT-AGCTATG)]2 cross-linked at the GC:GC site was determined by nuclear magnetic resonance (NMR). The deoxyguanosine-bridging cis-diammineplatinum(II) lies in the minor groove, and the complementary deoxycytidines are extrahelical. The double helix is locally reversed to a left-handed form, and the helix is unwound and bent toward the minor groove. These findings were independently confirmed by results from a phase-sensitive gel electrophoresis bending assay. The NMR structure differs markedly from previously proposed models but accounts for the chemical reactivity, the unwinding, and the bending of cis-DDP interstrand cross-linked DNA and may be important in the formation and repair of these cross-links in chromatin.
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[[Category: Hopkins PB]]
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[[Category: Huang H]]
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==About this Structure==
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[[Category: Reid BR]]
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1DDP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDP OCA].
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[[Category: Zhu L]]
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==Reference==
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Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8525382 8525382]
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[[Category: Protein complex]]
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[[Category: Drobny, G P.]]
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[[Category: Hopkins, P B.]]
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[[Category: Huang, H.]]
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[[Category: Reid, B R.]]
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[[Category: Zhu, L.]]
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[[Category: CPT]]
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[[Category: antitumor drug]]
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[[Category: cis-ddp]]
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[[Category: dna]]
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[[Category: double helix]]
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[[Category: interstrand cross-link]]
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[[Category: nmr]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:36:50 2008''
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Current revision

Solution structure of a CISPLATIN-INDUCED [CATAGCTATG]2 Interstrand cross-link

PDB ID 1ddp

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