4q7c

From Proteopedia

(Difference between revisions)

Current revision

Structure of AF2299, a CDP-alcohol phosphotransferase

Structural highlights

4q7c is a 2 chain structure with sequence from Archaeoglobus fulgidus DSM 4304. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.102Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O27985_ARCFU

Publication Abstract from PubMed

The CDP-alcohol phosphotransferase (CDP-AP) family of integral membrane enzymes catalyses the transfer of a substituted phosphate group from a CDP-linked donor to an alcohol acceptor. This is an essential reaction for phospholipid biosynthesis across all kingdoms of life, and it is catalysed solely by CDP-APs. Here we report the 2.0 A resolution crystal structure of a representative CDP-AP from Archaeoglobus fulgidus. The enzyme (AF2299) is a homodimer, with each protomer consisting of six transmembrane helices and an N-terminal cytosolic domain. A polar cavity within the membrane accommodates the active site, lined with the residues from an absolutely conserved CDP-AP signature motif (D(1)xxD(2)G(1)xxAR...G(2)xxxD(3)xxxD(4)). Structures in the apo, CMP-bound, CDP-bound and CDP-glycerol-bound states define functional roles for each of these eight conserved residues and allow us to propose a sequential, base-catalysed mechanism universal for CDP-APs, in which the fourth aspartate (D4) acts as the catalytic base.

Structural basis for catalysis in a CDP-alcohol phosphotransferase.,Sciara G, Clarke OB, Tomasek D, Kloss B, Tabuso S, Byfield R, Cohn R, Banerjee S, Rajashankar KR, Slavkovic V, Graziano JH, Shapiro L, Mancia F Nat Commun. 2014 Jun 13;5:4068. doi: 10.1038/ncomms5068. PMID:24923293^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sciara G, Clarke OB, Tomasek D, Kloss B, Tabuso S, Byfield R, Cohn R, Banerjee S, Rajashankar KR, Slavkovic V, Graziano JH, Shapiro L, Mancia F. Structural basis for catalysis in a CDP-alcohol phosphotransferase. Nat Commun. 2014 Jun 13;5:4068. doi: 10.1038/ncomms5068. PMID:24923293 doi:http://dx.doi.org/10.1038/ncomms5068

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4q7c"

@@ Line 1: / Line 1: @@
 ==Structure of AF2299, a CDP-alcohol phosphotransferase==
-<StructureSection load='4q7c' size='340' side='right' caption='[[4q7c]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
+<StructureSection load='4q7c' size='340' side='right'caption='[[4q7c]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4q7c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q7C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4q7c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q7C FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C2G:[CYTIDINE-5-PHOSPHATE]+GLYCERYLPHOSPHORIC+ACID+ESTER'>C2G</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:1-MONOOLEOYL-RAC-GLYCEROL'>MPG</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene><br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.102&#8491;</td></tr>
-<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4o6m|4o6m]], [[4o6n|4o6n]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C2G:[CYTIDINE-5-PHOSPHATE]+GLYCERYLPHOSPHORIC+ACID+ESTER'>C2G</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q7c RCSB], [http://www.ebi.ac.uk/pdbsum/4q7c PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7c OCA], [https://pdbe.org/4q7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q7c RCSB], [https://www.ebi.ac.uk/pdbsum/4q7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q7c ProSAT]</span></td></tr>
-<table>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/O27985_ARCFU O27985_ARCFU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4q7c" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Banerjee, S.]]
+[[Category: Archaeoglobus fulgidus DSM 4304]]
-[[Category: Clarke, O B.]]
+[[Category: Large Structures]]
-[[Category: Mancia, F.]]
+[[Category: Banerjee S]]
-[[Category: NYCOMPS, New York Consortium on Membrane Protein Structure.]]
+[[Category: Clarke OB]]
-[[Category: Rajashankar, K R.]]
+[[Category: Mancia F]]
-[[Category: Sciara, G.]]
+[[Category: Rajashankar KR]]
-[[Category: Shapiro, L.]]
+[[Category: Sciara G]]
-[[Category: Tomasek, D.]]
+[[Category: Shapiro L]]
-[[Category: Cdp-alcohol phosphotransferase]]
+[[Category: Tomasek D]]
-[[Category: Membrane protein]]
-[[Category: New york consortium on membrane protein structure]]
-[[Category: Nycomp]]
-[[Category: Psi-biology]]
-[[Category: Structural genomic]]
-[[Category: Transferase]]

4q7c

From Proteopedia

Current revision

Structure of AF2299, a CDP-alcohol phosphotransferase

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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