3j7p
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 3j7p is ON HOLD Authors: Voorhees, R.M., Fernandez, I.S., Scheres, S.H.W., Hegde, R.S. Description: Structure of the 80S mammalian ribosome bound t...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the 80S mammalian ribosome bound to eEF2== | |
| + | <SX load='3j7p' size='340' side='right' viewer='molstar' caption='[[3j7p]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3j7p]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4w21 4w21], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4w22 4w22], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4w23 4w23], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vx3 1vx3], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vx4 1vx4], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vx5 1vx5] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j74 3j74]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J7P FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7p OCA], [https://pdbe.org/3j7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j7p RCSB], [https://www.ebi.ac.uk/pdbsum/3j7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j7p ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/F2Z567_PIG F2Z567_PIG] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cotranslational protein translocation is a universally conserved process for secretory and membrane protein biosynthesis. Nascent polypeptides emerging from a translating ribosome are either transported across or inserted into the membrane via the ribosome-bound Sec61 channel. Here, we report structures of a mammalian ribosome-Sec61 complex in both idle and translating states, determined to 3.4 and 3.9 A resolution. The data sets permit building of a near-complete atomic model of the mammalian ribosome, visualization of A/P and P/E hybrid-state tRNAs, and analysis of a nascent polypeptide in the exit tunnel. Unprecedented chemical detail is observed for both the ribosome-Sec61 interaction and the conformational state of Sec61 upon ribosome binding. Comparison of the maps from idle and translating complexes suggests how conformational changes to the Sec61 channel could facilitate translocation of a secreted polypeptide. The high-resolution structure of the mammalian ribosome-Sec61 complex provides a valuable reference for future functional and structural studies. | ||
| - | + | Structure of the Mammalian ribosome-sec61 complex to 3.4 a resolution.,Voorhees RM, Fernandez IS, Scheres SH, Hegde RS Cell. 2014 Jun 19;157(7):1632-43. doi: 10.1016/j.cell.2014.05.024. Epub 2014 Jun , 12. PMID:24930395<ref>PMID:24930395</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3j7p" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Elongation factor 3D structures|Elongation factor 3D structures]] | ||
| + | *[[Ribosome 3D structures|Ribosome 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Sus scrofa]] | ||
| + | [[Category: Fernandez IS]] | ||
| + | [[Category: Hegde RS]] | ||
| + | [[Category: Scheres SHW]] | ||
| + | [[Category: Voorhees RM]] | ||
Current revision
Structure of the 80S mammalian ribosome bound to eEF2
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