This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


NAD synthase

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:58, 15 November 2021) (edit) (undo)
 
(18 intermediate revisions not shown.)
Line 1: Line 1:
-
+
<StructureSection load='' size='350' side='right' caption='NAD+ synthase complex with Mg+2 (green), ATP and deamino-NAD (PDB entry [[1xng]])' scene='54/541072/Cv/1'>
-
{{STRUCTURE_1ee1| PDB=1ee1 | SIZE=350| SCENE= |right|CAPTION= NAD+ synthase complex with Mg+2 (green), ATP (stick model) and deamino-NAD [[1ee1]] }}
+
== Function ==
-
 
+
'''NAD+ synthase''' or '''NH3-dependent NAD synthetase''' (NADS) catalyzes the conversion of ATP, deamino-NAD+ and NH<sub>3</sub> to AMP, NAD+ and diphosphate (PP). NADS is part of the NAD synthesis pathway<ref>PMID:12898714</ref> . See [[NAD]].
-
'''NAD+ synthase''' (NADS) catalyzes the conversion of ATP, deamino-NAD+ and NH3 to AMP, NAD+ and diphophate (PP). NADS is part of the NAD synthesis pathway.
+
 +
== Structural highlights ==
 +
The <scene name='54/541072/Cv/8'>ATP binding site</scene> contains <scene name='54/541072/Cv/9'>Mg+2 ion</scene>. The <scene name='54/541072/Cv/10'>NAD binding site is located in the interface of the 2 subunits</scene><ref>PMID:15645437</ref>. Water molecules are shown as red spheres.
 +
</StructureSection>
== 3D Structures of NAD+ synthase ==
== 3D Structures of NAD+ synthase ==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
 +
{{#tree:id=OrganizedByTopic|openlevels=0|
 +
 +
*'''NAD synthase'''
 +
 +
**[[3p52]] – NADS – ''Campylobacter jejuni'' <br />
 +
**[[3q4g]] – NADS (mutant) – ''Vibrio cholerae'' <br />
 +
**[[1wxf]] – EcNADS – ''Escherichia coli'' <br />
 +
**[[1xnh]] – HpNADS - ''Helicobacter pylori'' <br />
 +
**[[2pzb]] – BaNADS - ''Bacillus anthracis'' <br />
 +
**[[3dpi]] – NADS – ''Burkholderia pseudomallei''<br />
 +
**[[6kv3]] – NADS – ''Staphylococcus aureus''<br />
 +
**[[5wp0]] – NADS – ''Allivibrio fischeri''<br />
 +
**[[5huh]], [[5huj]] – NADS – ''Streptococcus pyogenes''<br />
 +
**[[3n05]] – NADS – ''Streptomyces avermitillis''<br />
 +
**[[4xfd]] – PaNADS – ''Pseudomonas aeruginosa''<br />
 +
**[[4q16]] – NADS – ''Deinococcus radiodurans''<br />
 +
**[[2e18]] – NADS – ''Pyrococcus horikoshii''<br />
 +
 +
*NADS binary complex
 +
 +
**[[1ifx]] – BsNADS + deamino-NAD – ''Bacillus subtilis'' <br />
 +
**[[1wxh]] – EcNADS + NAD <br />
 +
**[[3hmq]] – BsNADS + NAD – ''Salmonella typhimurium'' <br />
 +
**[[6c8q]] – NADS + NAD – ''Enterococcus faecalis''<br />
 +
**[[5f23]] – PaNADS + NAD <br />
 +
 +
*NADS ternary complex
-
[[3p52]] – NADS – ''Campylobacter jejuni'' <br />
+
**[[1ih8]] – BsNADS + Mg + ATP derivative <br />
-
[[3q4g]] – NADS (mutant) – ''Vibrio cholerae'' <br />
+
**[[1wxe]] – EcNADS + Mg + AMP <br />
-
[[1wxf]] – EcNADS – ''Escherichia coli'' <br />
+
**[[1wxg]] – EcNADS + Mg + deamino-NAD <br />
-
[[1xnh]] – HpNADS - ''Helicobacter pylori'' <br />
+
**[[2pz8]] – BaNADS + Mg + ATP derivative <br />
-
[[2pzb]] – BaNADS - ''Bacillus anthracis'' <br />
+
**[[3fiu]] – NADS + Mg + AMP – ''Francisella tularensis''<br />
-
[[3dpi]] – NADS – ''Burkholderia pseudomallei''<br />
+
-
===NADS binary complex===
+
*NADS quaternary complex
-
[[1ifx]] – BsNADS + deamino-NAD – ''Bacillus subtilis'' <br />
+
**[[1ee1]] – BsNADS + Mg + ATP + deamino-NAD <br />
-
[[1wxh]] – EcNADS + NAD <br />
+
**[[1fyd]] – BsNADS + Mg + AMP + PP <br />
-
[[3hmq]] – BsNADS + NAD – ''Salmonella typhimurium'' <br />
+
**[[1kqp]] – BsNADS + Mg + PP + NAD derivative<br />
 +
**[[1wxi]] – EcNADS + Mg + AMP + PP <br />
 +
**[[1xng]] – HpNADS + Mg + ATP + deamino-NAD <br />
 +
**[[2pza]] – BaNADS + Mg + AMP + PP <br />
-
===NADS ternary complex===
+
*NADS quinary complex
-
[[1ih8]] – BsNADS + Mg + ATP derivative <br />
+
**[[1nsy]] – BsNADS + Mg + ATP + ADP + PP <br />
-
[[1wxe]] – EcNADS + Mg + AMP <br />
+
**[[2nsy]] – BsNADS + Mg + AMP + NAD + PP <br />
-
[[1wxg]] – EcNADS + Mg + deamino-NAD <br />
+
-
[[2pz8]] – BaNADS + Mg + ATP derivative <br />
+
-
===NADS quaternary complex===
+
*NADS glutamine-dependent
-
[[1ee1]] – BsNADS + Mg + ATP + deamino-NAD <br />
+
**[[6ofb]] – hNADS + AMP + NAD derivative<br />
-
[[1fyd]] – BsNADS + Mg + AMP + PP <br />
+
**[[3sdb]] – MtNADS (mutant) – ''Mycobacterium tuberculosis'' <br />
-
[[1kqp]] – BsNADS + Mg + PP + NAD derivative<br />
+
**[[3seq]], [[3sez]] – MtNADS (mutant) + ATP derivative + pyridinium derivative <br />
-
[[1wxi]] – EcNADS + Mg + AMP + PP <br />
+
**[[3szg]] – MtNADS (mutant) + AMP + pyridinium derivative <br />
-
[[1xng]] – HpNADS + Mg + ATP + deamino-NAD <br />
+
**[[3syt]] – MtNADS + AMP + NAD + glutamate <br />
-
[[2pza]] – BaNADS + Mg + AMP + PP <br />
+
**[[3dla]] – MtNADS + norleucine + deamido-NAD <br />
 +
**[[6ofc]] – MtNADS (mutant) + AMP + sulfonamide derivative + glutamate <br />
 +
**[[4f4h]] – NADS – ''Burkholderia thailandensis''<br />
 +
**[[3ilv]] – NADS – ''Cytophaga hutchinsonii''<br />
-
[[1nsy]] – BsNADS + Mg + ATP + ADP + PP <br />
+
}}
-
[[2nsy]] – BsNADS + Mg + AMP + NAD + PP <br />
+
== References ==
 +
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

NAD+ synthase complex with Mg+2 (green), ATP and deamino-NAD (PDB entry 1xng)

Drag the structure with the mouse to rotate

3D Structures of NAD+ synthase

Updated on 15-November-2021

References

  1. ↑ Suda Y, Tachikawa H, Yokota A, Nakanishi H, Yamashita N, Miura Y, Takahashi N. Saccharomyces cerevisiae QNS1 codes for NAD(+) synthetase that is functionally conserved in mammals. Yeast. 2003 Aug;20(11):995-1005. PMID:12898714 doi:http://dx.doi.org/10.1002/yea.1008
  2. ↑ Kang GB, Kim YS, Im YJ, Rho SH, Lee JH, Eom SH. Crystal structure of NH3-dependent NAD+ synthetase from Helicobacter pylori. Proteins. 2005 Mar 1;58(4):985-8. PMID:15645437 doi:10.1002/prot.20377

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

Retrieved from "http://52.214.119.220/wiki/index.php/NAD_synthase"
Personal tools