1cfr

<StructureSection load='1cfr' size='340' side='right' caption='[[1cfr]], [[Resolution|resolution]] 2.15&Aring;' scene=''>

<table><tr><td colspan='2'>[[1cfr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFR FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cfr RCSB], [http://www.ebi.ac.uk/pdbsum/1cfr PDBsum]</span></td></tr>

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== Publication Abstract from PubMed ==

The X-ray crystal structure of Citrobacter freundii restriction endonuclease Cfr10I has been determined at a resolution of 2.15 A by multiple isomorphous replacement methods and refined to an R-factor of 19.64%. The structure of Cfr10I represents the first structure of a restriction endonuclease recognizing a degenerated nucleotide sequence. Structural comparison of Cfr10I with previously solved structures of other restriction enzymes suggests that recognition of specific sequence occurs through contacts in the major and the minor grooves of DNA. The arrangement of the putative active site residues shows some striking differences from previously described restriction endonucleases and supports a two-metal-ion mechanism of catalysis.

 

Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15 A resolution.,Bozic D, Grazulis S, Siksnys V, Huber R J Mol Biol. 1996 Jan 12;255(1):176-86. PMID:8568865<ref>PMID:8568865</ref>

 

*[[Endonuclease|Endonuclease]]

[[Category: Type II site-specific deoxyribonuclease]]

[[Category: Bozic, D.]]

[[Category: Grazulis, S.]]

[[Category: Huber, R.]]

[[Category: Siksnys, V.]]

[[Category: Restriction enzyme]]