2mkc
From Proteopedia
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==Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex== | ==Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex== | ||
| - | <StructureSection load='2mkc' size='340' side='right' caption='[[2mkc | + | <StructureSection load='2mkc' size='340' side='right'caption='[[2mkc]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2mkc]] is a 3 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MKC OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[2mkc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MKC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MKC FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <table> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mkc OCA], [https://pdbe.org/2mkc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mkc RCSB], [https://www.ebi.ac.uk/pdbsum/2mkc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mkc ProSAT]</span></td></tr> |
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/IST3_YEAST IST3_YEAST] Required for pre-mRNA splicing and spliceosome assembly. As part of the pre-mRNA retention and splicing (RES) complex, required for nuclear pre-mRNA retention and efficient splicing. Required for MER1-activated splicing.<ref>PMID:11287609</ref> <ref>PMID:15565172</ref> <ref>PMID:14973223</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The precursor mRNA (pre-mRNA) retention and splicing (RES) complex is a spliceosomal complex that is present in yeast and humans and is important for RNA splicing and retention of unspliced pre-mRNA. Here, we present the solution NMR structure of the RES core complex from Saccharomyces cerevisiae. Complex formation leads to an intricate folding of three components-Snu17p, Bud13p and Pml1p-that stabilizes the RNA-recognition motif (RRM) fold of Snu17p and increases binding affinity in tertiary interactions between the components by more than 100-fold compared to that in binary interactions. RES interacts with pre-mRNA within the spliceosome, and through the assembly of the RES core complex RNA binding efficiency is increased. The three-dimensional structure of the RES core complex highlights the importance of cooperative folding and binding in the functional organization of the spliceosome. | ||
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| + | Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex.,Wysoczanski P, Schneider C, Xiang S, Munari F, Trowitzsch S, Wahl MC, Luhrmann R, Becker S, Zweckstetter M Nat Struct Mol Biol. 2014 Sep 14. doi: 10.1038/nsmb.2889. PMID:25218446<ref>PMID:25218446</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2mkc" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Becker | + | [[Category: Large Structures]] |
| - | [[Category: Luhrmann | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Munari | + | [[Category: Becker S]] |
| - | [[Category: Schneider | + | [[Category: Luhrmann R]] |
| - | [[Category: Trowitzsch | + | [[Category: Munari F]] |
| - | [[Category: Wahl | + | [[Category: Schneider C]] |
| - | [[Category: Wysoczanski | + | [[Category: Trowitzsch S]] |
| - | [[Category: Xiang | + | [[Category: Wahl MC]] |
| - | [[Category: Zweckstetter | + | [[Category: Wysoczanski P]] |
| - | + | [[Category: Xiang S]] | |
| - | + | [[Category: Zweckstetter M]] | |
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Current revision
Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex
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