1e5d

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[[Image:1e5d.jpg|left|200px]]
 
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{{Structure
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==RUBREDOXIN OXYGEN:OXIDOREDUCTASE (ROO) FROM ANAEROBE DESULFOVIBRIO GIGAS==
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|PDB= 1e5d |SIZE=350|CAPTION= <scene name='initialview01'>1e5d</scene>, resolution 2.50&Aring;
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<StructureSection load='1e5d' size='340' side='right'caption='[[1e5d]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE= <scene name='pdbsite=FEA:Diiron+Redox+Catalytic+Site+For+Direct+Dioxygen+To+Water+...'>FEA</scene>, <scene name='pdbsite=FEB:Diiron+Redox+Catalytic+Site+For+Direct+Dioxygen+To+Water+...'>FEB</scene>, <scene name='pdbsite=FMA:Fmn+Binding+Site+For+Chain+A'>FMA</scene> and <scene name='pdbsite=FMB:Fmn+Binding+Site+For+Chain+B'>FMB</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene> and <scene name='pdbligand=OXY:OXYGEN MOLECULE'>OXY</scene>
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<table><tr><td colspan='2'>[[1e5d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Megalodesulfovibrio_gigas Megalodesulfovibrio gigas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E5D FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e5d OCA], [https://pdbe.org/1e5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e5d RCSB], [https://www.ebi.ac.uk/pdbsum/1e5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e5d ProSAT]</span></td></tr>
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</table>
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'''RUBREDOXIN OXYGEN:OXIDOREDUCTASE (ROO) FROM ANAEROBE DESULFOVIBRIO GIGAS'''
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== Function ==
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[https://www.uniprot.org/uniprot/ROO_MEGG1 ROO_MEGG1] Catalyzes the four-electron reduction of one oxygen molecule to two water molecules.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e5/1e5d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e5d ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Desulfovibrio gigas is a strict anaerobe that contains a well-characterized metabolic pathway that enables it to survive transient contacts with oxygen. The terminal enzyme in this pathway, rubredoxin:oxygen oxidoreductase (ROO) reduces oxygen to water in a direct and safe way. The 2.5 A resolution crystal structure of ROO shows that each monomer of this homodimeric enzyme consists of a novel combination of two domains, a flavodoxin-like domain and a Zn-beta-lactamase-like domain that contains a di-iron center for dioxygen reduction. This is the first structure of a member of a superfamily of enzymes widespread in strict and facultative anaerobes, indicating its broad physiological significance.
Desulfovibrio gigas is a strict anaerobe that contains a well-characterized metabolic pathway that enables it to survive transient contacts with oxygen. The terminal enzyme in this pathway, rubredoxin:oxygen oxidoreductase (ROO) reduces oxygen to water in a direct and safe way. The 2.5 A resolution crystal structure of ROO shows that each monomer of this homodimeric enzyme consists of a novel combination of two domains, a flavodoxin-like domain and a Zn-beta-lactamase-like domain that contains a di-iron center for dioxygen reduction. This is the first structure of a member of a superfamily of enzymes widespread in strict and facultative anaerobes, indicating its broad physiological significance.
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==About this Structure==
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Structure of a dioxygen reduction enzyme from Desulfovibrio gigas.,Frazao C, Silva G, Gomes CM, Matias P, Coelho R, Sieker L, Macedo S, Liu MY, Oliveira S, Teixeira M, Xavier AV, Rodrigues-Pousada C, Carrondo MA, Le Gall J Nat Struct Biol. 2000 Nov;7(11):1041-5. PMID:11062560<ref>PMID:11062560</ref>
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1E5D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_gigas Desulfovibrio gigas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E5D OCA].
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==Reference==
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Structure of a dioxygen reduction enzyme from Desulfovibrio gigas., Frazao C, Silva G, Gomes CM, Matias P, Coelho R, Sieker L, Macedo S, Liu MY, Oliveira S, Teixeira M, Xavier AV, Rodrigues-Pousada C, Carrondo MA, Le Gall J, Nat Struct Biol. 2000 Nov;7(11):1041-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11062560 11062560]
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[[Category: Desulfovibrio gigas]]
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[[Category: Single protein]]
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[[Category: Carrondo, M A.]]
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[[Category: Coelho, R.]]
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[[Category: Frazao, C.]]
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[[Category: Gall, J Le.]]
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[[Category: Gomes, C M.]]
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[[Category: Liu, M Y.]]
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[[Category: Macedo, S.]]
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[[Category: Matias, P.]]
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[[Category: Oliveira, S.]]
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[[Category: Rodrigues-Pousada, C.]]
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[[Category: Sieker, L.]]
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[[Category: Silva, G.]]
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[[Category: Teixeira, M.]]
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[[Category: Xavier, A V.]]
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[[Category: FEO]]
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[[Category: FMN]]
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[[Category: OXY]]
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[[Category: diiron-centre]]
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[[Category: flavoprotein]]
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[[Category: lactamase-fold]]
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[[Category: oxidoreductase]]
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[[Category: oxygenreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:49:58 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1e5d" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Megalodesulfovibrio gigas]]
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[[Category: Carrondo MA]]
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[[Category: Coelho R]]
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[[Category: Frazao C]]
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[[Category: Gomes CM]]
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[[Category: Le Gall J]]
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[[Category: Liu MY]]
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[[Category: Macedo S]]
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[[Category: Matias P]]
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[[Category: Oliveira S]]
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[[Category: Rodrigues-Pousada C]]
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[[Category: Sieker L]]
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[[Category: Silva G]]
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[[Category: Teixeira M]]
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[[Category: Xavier AV]]

Current revision

RUBREDOXIN OXYGEN:OXIDOREDUCTASE (ROO) FROM ANAEROBE DESULFOVIBRIO GIGAS

PDB ID 1e5d

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