User:Avinash S. Punekar/Sandbox 1
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< User:Avinash S. Punekar(Difference between revisions)
(New page: ==Structure of 23S rRNA methyltransferase RlmJ== <StructureSection load='4blu' size='340' side='right' caption='Methyltransferase RlmJ' scene=''> This is a default text for your page '''Av...) |
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| - | == | + | === Introduction === |
| - | + | Post-transcriptional modification of ribosomal RNA (rRNA) occurs in all living organisms. In bacteria, rRNA modifications are done by site-specific enzymes. One such rRNA modification enzyme is a S-adenosyl-L-methionine (SAM) dependent methyltransferase RlmJ. The biochemical experiments showed that the ''Escherichia coli'' RlmJ transfers a methyl group from SAM to the exocyclic nitrogen N6 of adenosine A2030 (m6A2030) in the 23S rRNA <ref>PMID:22847818</ref>. | |
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| - | == | + | <StructureSection load='4BLU_chain_A.pdb' size='340' side='right' caption='Methyltransferase RlmJ' scene='59/598869/Rlmj_landing_view/1'> |
| + | === Overall structure === | ||
| + | The ''E. coli'' RlmJ structure consists of a <scene name='59/598869/Rlmj_helical_subdomain/1'>helical subdomain</scene> (HS, residues 47-98, colour: <font color='orange'><b>orange</b></font>) inserted within the main <scene name='59/598869/Rlmj_mtase_domain/1'>methyltransferase domain</scene> (MTase, residues 1-46 and 99-280, colour: <font color='blue'><b>blue</b></font>). | ||
| - | == | + | === Structural rearrangement upon cofactor and substrate binding === |
| - | == | + | === Proposed catalytic mechanism === |
| - | == Structural highlights == | ||
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| - | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Current revision
Introduction
Post-transcriptional modification of ribosomal RNA (rRNA) occurs in all living organisms. In bacteria, rRNA modifications are done by site-specific enzymes. One such rRNA modification enzyme is a S-adenosyl-L-methionine (SAM) dependent methyltransferase RlmJ. The biochemical experiments showed that the Escherichia coli RlmJ transfers a methyl group from SAM to the exocyclic nitrogen N6 of adenosine A2030 (m6A2030) in the 23S rRNA [1].
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References
- ↑ Golovina AY, Dzama MM, Osterman IA, Sergiev PV, Serebryakova MV, Bogdanov AA, Dontsova OA. The last rRNA methyltransferase of E. coli revealed: the yhiR gene encodes adenine-N6 methyltransferase specific for modification of A2030 of 23S ribosomal RNA. RNA. 2012 Sep;18(9):1725-34. doi: 10.1261/rna.034207.112. Epub 2012 Jul 30. PMID:22847818 doi:10.1261/rna.034207.112
