This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
User:Avinash S. Punekar/Sandbox 1
From Proteopedia
| (7 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
=== Introduction === | === Introduction === | ||
| - | Post-transcriptional modification of ribosomal RNA (rRNA) occurs in all living organisms. In bacteria, rRNA modifications are done by site-specific enzymes. One such rRNA modification enzyme is a S-adenosyl-L-methionine (SAM) dependent methyltransferase RlmJ. The biochemical experiments showed that the ''Escherichia coli'' RlmJ transfers a methyl group from SAM to the exocyclic nitrogen N6 of adenosine A2030 (m6A2030) in the 23S rRNA. | + | Post-transcriptional modification of ribosomal RNA (rRNA) occurs in all living organisms. In bacteria, rRNA modifications are done by site-specific enzymes. One such rRNA modification enzyme is a S-adenosyl-L-methionine (SAM) dependent methyltransferase RlmJ. The biochemical experiments showed that the ''Escherichia coli'' RlmJ transfers a methyl group from SAM to the exocyclic nitrogen N6 of adenosine A2030 (m6A2030) in the 23S rRNA <ref>PMID:22847818</ref>. |
<StructureSection load='4BLU_chain_A.pdb' size='340' side='right' caption='Methyltransferase RlmJ' scene='59/598869/Rlmj_landing_view/1'> | <StructureSection load='4BLU_chain_A.pdb' size='340' side='right' caption='Methyltransferase RlmJ' scene='59/598869/Rlmj_landing_view/1'> | ||
| - | This is a default text for your page '''Avinash S. Punekar/Sandbox 1'''. Click above on '''edit this page''' to modify. Be careful with the < and > signs. | ||
| - | You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | ||
| - | |||
=== Overall structure === | === Overall structure === | ||
| + | The ''E. coli'' RlmJ structure consists of a <scene name='59/598869/Rlmj_helical_subdomain/1'>helical subdomain</scene> (HS, residues 47-98, colour: <font color='orange'><b>orange</b></font>) inserted within the main <scene name='59/598869/Rlmj_mtase_domain/1'>methyltransferase domain</scene> (MTase, residues 1-46 and 99-280, colour: <font color='blue'><b>blue</b></font>). | ||
=== Structural rearrangement upon cofactor and substrate binding === | === Structural rearrangement upon cofactor and substrate binding === | ||
| Line 12: | Line 10: | ||
=== Proposed catalytic mechanism === | === Proposed catalytic mechanism === | ||
| - | == Structural highlights == | ||
| - | |||
| - | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Current revision
Introduction
Post-transcriptional modification of ribosomal RNA (rRNA) occurs in all living organisms. In bacteria, rRNA modifications are done by site-specific enzymes. One such rRNA modification enzyme is a S-adenosyl-L-methionine (SAM) dependent methyltransferase RlmJ. The biochemical experiments showed that the Escherichia coli RlmJ transfers a methyl group from SAM to the exocyclic nitrogen N6 of adenosine A2030 (m6A2030) in the 23S rRNA [1].
| |||||||||||
References
- ↑ Golovina AY, Dzama MM, Osterman IA, Sergiev PV, Serebryakova MV, Bogdanov AA, Dontsova OA. The last rRNA methyltransferase of E. coli revealed: the yhiR gene encodes adenine-N6 methyltransferase specific for modification of A2030 of 23S ribosomal RNA. RNA. 2012 Sep;18(9):1725-34. doi: 10.1261/rna.034207.112. Epub 2012 Jul 30. PMID:22847818 doi:10.1261/rna.034207.112
