4a9v

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==Pseudomonas fluorescens PhoX==
==Pseudomonas fluorescens PhoX==
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<StructureSection load='4a9v' size='340' side='right' caption='[[4a9v]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
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<StructureSection load='4a9v' size='340' side='right'caption='[[4a9v]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4a9v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Psepf Psepf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A9V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A9V FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4a9v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens_Pf0-1 Pseudomonas fluorescens Pf0-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A9V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A9V FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zwu|3zwu]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a9v OCA], [https://pdbe.org/4a9v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a9v RCSB], [https://www.ebi.ac.uk/pdbsum/4a9v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a9v ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a9v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a9v RCSB], [http://www.ebi.ac.uk/pdbsum/4a9v PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q3K5N8_PSEPF Q3K5N8_PSEPF]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Alkaline phosphatases play a crucial role in phosphate acquisition by microorganisms. To expand our understanding of catalysis by this class of enzymes, we have determined the structure of the widely occurring microbial alkaline phosphatase PhoX. The enzyme contains a complex active-site cofactor comprising two antiferromagnetically coupled ferric iron ions (Fe(3+)), three calcium ions (Ca(2+)), and an oxo group bridging three of the metal ions. Notably, the main part of the cofactor resembles synthetic oxide-centered triangular metal complexes. Structures of PhoX-ligand complexes reveal how the active-site metal ions bind substrate and implicate the cofactor oxo group in the catalytic mechanism. The presence of iron in PhoX raises the possibility that iron bioavailability limits microbial phosphate acquisition.
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A complex iron-calcium cofactor catalyzing phosphotransfer chemistry.,Yong SC, Roversi P, Lillington J, Rodriguez F, Krehenbrink M, Zeldin OB, Garman EF, Lea SM, Berks BC Science. 2014 Sep 5;345(6201):1170-3. doi: 10.1126/science.1254237. PMID:25190793<ref>PMID:25190793</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4a9v" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
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*[[Alkaline phosphatase|Alkaline phosphatase]]
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*[[Alkaline phosphatase 3D structures|Alkaline phosphatase 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Alkaline phosphatase]]
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[[Category: Large Structures]]
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[[Category: Psepf]]
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[[Category: Pseudomonas fluorescens Pf0-1]]
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[[Category: Berks, B C.]]
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[[Category: Berks BC]]
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[[Category: Garman, E F.]]
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[[Category: Garman EF]]
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[[Category: Lea, S M.]]
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[[Category: Lea SM]]
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[[Category: Lillington, J E.D.]]
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[[Category: Lillington JED]]
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[[Category: Roversi, P.]]
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[[Category: Roversi P]]
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[[Category: Yong, S C.]]
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[[Category: Yong SC]]
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[[Category: Zeldin, O B.]]
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[[Category: Zeldin OB]]
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[[Category: Beta-propeller]]
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[[Category: Hydrolase]]
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Current revision

Pseudomonas fluorescens PhoX

PDB ID 4a9v

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